Protein Purification
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Cytiva HiScreen™ Capto™ Q ImpRes and Capto™ SP ImpRes
High-resolution intermediate purification and polishing based on the well-established Capto platform with traditional ligands
| Particle Size | 36 to 44μm |
|---|---|
| Buffer | 1M NaOH, 8M Urea, 6M Guanidine Hydrochloride, 30% Isopropanol and 70% Ethanol |
| Flow Rate | 220 cm/hr. |
| Height (Metric) | 200mm |
| Content And Storage | 4° to 30°C |
| Diameter (English) Inner | 39.3 in. |
| Diameter (Metric) Inner | 1000mm |
| dimMatrix | Capto Q Chromatography Media |
| Quantity | 100 mL |
| For Use With (Application) | High-throughput intermediate purification and polishing steps of a wide range of biomolecules |
| Support Type | High-flow Agarose |
| Max. Pressure | <3 bar |
| Height (English) | 7.87 in. |
Cytiva Sephadex™ G-25 Media
Use of Sephadex™ is well established as a gel filtration medium for desalting and buffer exchange in industrial applications. Cytiva Life Sciences™ Sephadex™ G-25 Media quickly desalts, removes contaminants and transfers to a new buffer in a single step.
| Height (Metric) | 30cm |
|---|---|
| Content And Storage | 4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH |
| Bed Volume | 4 to 6mL/g in Distilled Water |
| Format | Solid Spherical Bead |
| pH Range | 2 to 13 |
| Product Type | Sephadex G-25 Media |
| Diameter (Metric) Inner | 2.6cm |
| For Use With (Application) | Desalting and Buffer exchange and removal free label in industrial applications |
| Support Type | Cross-linked Dextran |
| Height (English) | 11.81 in. |
Cytiva Ni Sepharose™ 6 Fast Flow
Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.
| Particle Size | 90μm |
|---|---|
| Buffer | 0.1M HCl, 0.1M NaOH, 8M Urea |
| Flow Rate | 600 cm/hr. |
| Height (Metric) | 250mm |
| Content And Storage | 4° to 30°C |
| pH Range | 3 to 12 |
| Product Type | Ni Sepharose 6 FF chromatography media |
| dimMatrix | Ni-Sepharose |
| Support Type | 6% Cross-linked Agarose |
| Max. Pressure | 1 bar |
| Height (English) | 9.84 in. |
Cytiva Sephacryl™ High Resolution Media
Purify proteins, polysaccharides, and other macromolecules by gel filtration at laboratory and industrial scale. Cytiva Life Sciences™Sephacryl™ High Resolution Media enables fast and reproducible purifications.
| Buffer | 0.2M NaOH, 0.1M HCl, 1M Acetic Acid, 8M Urea, 6M Guanidine HCl, 1% SDS, 20% Ethanol, 30% Propanol, 30% Aceto nitrile (tested at 40°C for 1 week), 0.5M NaOH (only for cleaning-in-place) |
|---|---|
| Height (Metric) | 15cm |
| Content And Storage | 4° to 30°C |
| pH Range | 3 to 11 |
| dimMatrix | Gel Filtration Media |
| For Use With (Application) | Purification of Proteins, Polysaccharides and other macromolecules |
| Support Type | Allyl Dextran and N, N'-methylenebisacrylamide |
| Max. Pressure | 1 bar |
| Height (English) | 5.9 in. |
Cytiva Ni Sepharose™ excel
Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
| Particle Size | 90μm |
|---|---|
| Buffer | 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol |
| Flow Rate | 150 to 600cm/hr. |
| Content And Storage | 4° to 30°C |
| pH Range | 2 to 12 |
| Product Type | Ni Sepharose excel chromato graphy media |
| dimMatrix | Ni-Sepharose |
| Support Type | 6% Highly Cross-linked Agarose |
Cytiva Sephadex™ G-25 DNA Grade SF Cleanup Gel
Tested to ensure reproducibly high recovery of DNA (10 bases in length) from small molecules by gel filtration
Cytiva HiScreen Phenyl FF (High Sub)
HiScreen Phenyl FF (high sub) columns are prepacked with Phenyl Sepharose 6 Fast Flow hydrophobic interaction chromatography (HIC) media. The columns are an excellent choice for method optimization and parameter screening.
Cytiva Capto™ Core 700
Capto Core 700 is designed for intermediate purification and polishing of viruses and other large biomolecules.
| Particle Size | 90μm |
|---|---|
| Buffer | 1M NaOH, 6M Guanidine Hydrochloride, 30% Isopropanol and 70% Ethanol |
| Ligand Type | Octylamine |
| Flow Rate | <500 cm/hr. |
| Content And Storage | 4° to 30°C |
| pH Range | 2 to 14 |
| Product Type | Capto Core 700 chromato graphy media |
| dimMatrix | Multimodal Resin |
| For Use With (Application) | Intermediate purification and polishing of viruses and other large biomolecules |
| Support Type | Highly Cross-linked Agarose |
Cytiva HiScreen™ Capto™ adhere Multimodal Prepacked Column
For intermediate purification and polishing of monoclonal antibodies after the protein A capture step
Cytiva HiScreen™ Blue, Ni and DEAE Sepharose™ Prepacked Fast Flow Columns
Used for method optimization and parameter screening
| Particle Size | 45 to 165μm |
|---|---|
| Height (Metric) | 100mm |
| Diameter (English) Inner | 0.3 in. |
| Bed Volume | 4.7 mL |
| Diameter (Metric) Inner | 7.7 mm |
| Quantity | 1 x 4.7mL |
| Material | Polypropylene |
| Support Type | 6% Cross-linked Agarose |
| Max. Pressure | 1.5 bar |
| Height (English) | 3.93 in. |
Cytiva HiPrep™ IEX FF 16/10 Columns
Provide fast, reliable and reproducible preparative ion exchange separations
| Buffer | 1M NaOH, 8M Urea, 8M Guanidine Hydrochloride, 70% Ethanol |
|---|---|
| Height (Metric) | 100mm |
| Content And Storage | 4° to 30°C |
| Diameter (English) Inner | 0.62 in. |
| Bed Volume | 20mL |
| Column Type | Ion Exchange Column |
| Diameter (Metric) Inner | 16mm |
| Quantity | 1 x 20mL |
| Material | Polypropylene |
| For Use With (Application) | Preparative separations of Proteins and other biomolecules using ion exchange (IEX) chromato graphy |
| Particle Size | 45 to 165μm |
| Flow Rate | >300 cm/hr. |
| Support Type | 6% Cross-linked Agarose |
| Height (English) | 3.93 in. |
Cytiva HiScreen™ Capto™ MMC Multimodal Prepacked Column
Allows binding of proteins at the conductivity of the feed material and solves specific purification challenges
Cytiva HiTrap™ Capto™ Core 700 Columns
HiTrap Capto Core 700 is prepacked with Capto Core 700, a multimodal BioProcess medium for intermediate purification and polishing of viruses and other large biomolecules.
Cytiva HiScreen Capto™ Core 700 Columns
Capto Core 700 is designed for intermediate purification and polishing of viruses and other large biomolecules.
Cytiva HiScreen™ Q Sepharose™ and SP Sepharose™ Fast Flow IEX Columns
Designed for method optimization and parameter screening
| Buffer | 1M NaOH, 8M Urea, 8M Guanidine Hydrochloride, 70% Ethanol |
|---|---|
| Height (Metric) | 100mm |
| Content And Storage | 4° to 30°C |
| Diameter (English) Inner | 0.3 in. |
| Bed Volume | 4.7mL |
| Column Type | Ion Exchange Column |
| Diameter (Metric) Inner | 7.7mm |
| Quantity | 1 x 4.7mL |
| Material | Polypropylene |
| For Use With (Application) | Optimization and parameter screening, ion exchange chromato graphy |
| Max. Pressure | 1.5 bar |
| Particle Size | 45 to 165μm |
| Flow Rate | <450 cm/hr. |
| Support Type | 6% Cross-linked Agarose |
| Height (English) | 3.93 in. |