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Protein Purification Reagents and Kits

A variety of reagents, columns, kits, and associated products designed for high-yield purification of proteins.
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1 – 15 of 31 results
1

Cytiva HiScreen™ Capto™ Q ImpRes and Capto™ SP ImpRes

High-resolution intermediate purification and polishing based on the well-established Capto platform with traditional ligands

EDGE
Particle Size 36 to 44μm
Buffer 1M NaOH, 8M Urea, 6M Guanidine Hydrochloride, 30% Isopropanol and 70% Ethanol
Flow Rate 220 cm/hr.
Height (Metric) 200mm
Content And Storage 4° to 30°C
Diameter (English) Inner 39.3 in.
Diameter (Metric) Inner 1000mm
dimMatrix Capto Q Chromatography Media
Quantity 100 mL
For Use With (Application) High-throughput intermediate purification and polishing steps of a wide range of biomolecules
Support Type High-flow Agarose
Max. Pressure <3 bar
Height (English) 7.87 in.
2

Cytiva Ni Sepharose™ 6 Fast Flow

Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.

Particle Size 90μm
Buffer 0.1M HCl, 0.1M NaOH, 8M Urea
Flow Rate 600 cm/hr.
Height (Metric) 250mm
Content And Storage 4° to 30°C
pH Range 3 to 12
Product Type Ni Sepharose 6 FF chromatography media
dimMatrix Ni-Sepharose
Support Type 6% Cross-linked Agarose
Max. Pressure 1 bar
Height (English) 9.84 in.
3

Cytiva Sephacryl™ High Resolution Media

Purify proteins, polysaccharides, and other macromolecules by gel filtration at laboratory and industrial scale. Cytiva Life Sciences™Sephacryl™ High Resolution Media enables fast and reproducible purifications.

Buffer 0.2M NaOH, 0.1M HCl, 1M Acetic Acid, 8M Urea, 6M Guanidine HCl, 1% SDS, 20% Ethanol, 30% Propanol, 30% Aceto nitrile (tested at 40°C for 1 week), 0.5M NaOH (only for cleaning-in-place)
Height (Metric) 15cm
Content And Storage 4° to 30°C
pH Range 3 to 11
dimMatrix Gel Filtration Media
For Use With (Application) Purification of Proteins, Polysaccharides and other macromolecules
Support Type Allyl Dextran and N, N'-methylenebisacrylamide
Max. Pressure 1 bar
Height (English) 5.9 in.
4

Cytiva Ni Sepharose™ excel

Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

Particle Size 90μm
Buffer 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol
Flow Rate 150 to 600cm/hr.
Content And Storage 4° to 30°C
pH Range 2 to 12
Product Type Ni Sepharose excel chromato graphy media
dimMatrix Ni-Sepharose
Support Type 6% Highly Cross-linked Agarose
5

Cytiva HiScreen Phenyl FF (High Sub)

HiScreen Phenyl FF (high sub) columns are prepacked with Phenyl Sepharose 6 Fast Flow hydrophobic interaction chromatography (HIC) media. The columns are an excellent choice for method optimization and parameter screening.

6

Cytiva Capto™ Core 700

Capto Core 700 is designed for intermediate purification and polishing of viruses and other large biomolecules.

Particle Size 90μm
Buffer 1M NaOH, 6M Guanidine Hydrochloride, 30% Isopropanol and 70% Ethanol
Ligand Type Octylamine
Flow Rate <500 cm/hr.
Content And Storage 4° to 30°C
pH Range 2 to 14
Product Type Capto Core 700 chromato graphy media
dimMatrix Multimodal Resin
For Use With (Application) Intermediate purification and polishing of viruses and other large biomolecules
Support Type Highly Cross-linked Agarose
7

Cytiva HiScreen™ Capto™ adhere Multimodal Prepacked Column

For intermediate purification and polishing of monoclonal antibodies after the protein A capture step

8

Cytiva HiScreen™ Blue, Ni and DEAE Sepharose™ Prepacked Fast Flow Columns

Used for method optimization and parameter screening

Particle Size 45 to 165μm
Height (Metric) 100mm
Diameter (English) Inner 0.3 in.
Bed Volume 4.7 mL
Diameter (Metric) Inner 7.7 mm
Quantity 1 x 4.7mL
Material Polypropylene
Support Type 6% Cross-linked Agarose
Max. Pressure 1.5 bar
Height (English) 3.93 in.
9

Cytiva HiTrap™ Capto™ Core 700 Columns

HiTrap Capto Core 700 is prepacked with Capto Core 700, a multimodal BioProcess medium for intermediate purification and polishing of viruses and other large biomolecules.

10

Cytiva HiPrep™ IEX FF 16/10 Columns

Provide fast, reliable and reproducible preparative ion exchange separations

Buffer 1M NaOH, 8M Urea, 8M Guanidine Hydrochloride, 70% Ethanol
Height (Metric) 100mm
Content And Storage 4° to 30°C
Diameter (English) Inner 0.62 in.
Bed Volume 20mL
Column Type Ion Exchange Column
Diameter (Metric) Inner 16mm
Quantity 1 x 20mL
Material Polypropylene
For Use With (Application) Preparative separations of Proteins and other biomolecules using ion exchange (IEX) chromato graphy
Particle Size 45 to 165μm
Flow Rate >300 cm/hr.
Support Type 6% Cross-linked Agarose
Height (English) 3.93 in.
11

Cytiva HiScreen™ Q Sepharose™ and SP Sepharose™ Fast Flow IEX Columns

Designed for method optimization and parameter screening

Buffer 1M NaOH, 8M Urea, 8M Guanidine Hydrochloride, 70% Ethanol
Height (Metric) 100mm
Content And Storage 4° to 30°C
Diameter (English) Inner 0.3 in.
Bed Volume 4.7mL
Column Type Ion Exchange Column
Diameter (Metric) Inner 7.7mm
Quantity 1 x 4.7mL
Material Polypropylene
For Use With (Application) Optimization and parameter screening, ion exchange chromato graphy
Max. Pressure 1.5 bar
Particle Size 45 to 165μm
Flow Rate <450 cm/hr.
Support Type 6% Cross-linked Agarose
Height (English) 3.93 in.
12

Cytiva Chelating Sepharose™ Fast Flow Medium

Use for purifying native and recombinant proteins and peptides that have an affinity for metal ions. Chelating Sepharose™ Fast Flow Medium provides robust performance and regulatory support.

13

Cytiva HiScreen™ Capto™ MMC Multimodal Prepacked Column

Allows binding of proteins at the conductivity of the feed material and solves specific purification challenges

14

Cytiva PD MidiTrap™ G-10

Cleanup biological samples, such as peptides, small proteins, and oligosaccharides. Cytiva Life Sciences™ PD MidiTrap™ G-10 offers fast desalting and efficient removal of contaminants such as salts, dyes, and radioactive labels.

15

Cytiva HiPrep™ IMAC FF 16/10 Column

For optimizing purification of histidine-tagged proteins at laboratory scale