Cell Based Assays
Molecular Probes™ pHrodo™ Red and Green Epidermal Growth Factor (EGF) and pHrodo™ Red Transferrin Conjugates
Detect internalized EGF and transferrin in cells with the pHrodo Red and pHrodo Green Epidermal Growth Factor (EGF) and pHrodo Red Transferrin conjugates, for use in applications such as HCS, flow cytometry, microplate-based fluorometry, and HTS.
Invitrogen™ Image-iT™ Green Hypoxia Reagent
Image-iT Green Hypoxia Reagent is a novel, fixable fluorogenic compound for measuring hypoxia in live cells.
Invitrogen™ Dextran, Cascade Blue™, 3000 MW, Anionic, Lysine Fixable
Rigorous methods for removing as much unconjugated dye as practical, followed by assay of dextran conjugates by thin-layer chromatography to help ensure absence of low molecular weight contaminants
Invitrogen™ Fura Red™, AM, cell permeant
Visible light-excitable fura-2 analog that offers particular possibilities for ratiometric measurement of Ca2+ in single cells
Invitrogen™ Fluorescein Diacetate (FDA)
Cell-permeant esterase substrate that can serve as viability probe
Invitrogen™ EnzChek™ Reverse Transcriptase Assay Kit
The EnzChek Reverse Transcriptase Assay Kit is a convenient, efficient, and inexpensive assay for measuring reverse transcriptase activity and screening reverse transcriptase inhibitors.
Molecular Probes™ MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)
MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)
Invitrogen™ Griess Reagent Kit, for nitrite quantitation
For nitrite quantitation
Invitrogen™ LIVE/DEAD™ Fixable Yellow Dead Cell Stain Kit, for 405 nm excitation
The LIVE/DEAD™ Fixable Yellow Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining, or prior to elimination of biohazardous materials using formaldehyde fixation.
Invitrogen™ eBioscience™ 7-AAD Viability Staining Solution
Ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. Can be used in combination with PE- and FITC-conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is that there is minimal spectral overlap between these emissions.
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 506
A viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation, and/or permeabilization procedures.
Invitrogen™ eBioscience™ Propidium Iodide
Standard reagent used for assessing cell viability and exclusion of non-viable cells in flow cytometry. PI binds to double-stranded DNA, but is excluded from cells with intact plasma membranes.
Invitrogen™ CyQUANT™ LDH Cytotoxicity Assays
CyQUANT cytotoxicity assays measure cellular toxicity based on the release of cytosolic enzymes (LDH or G6PD) from damaged cells. These cytotoxicity assays utilize either colorimetric or fluorescence detection and allow continuous monitoring of cell death using microplate readers.