Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
TAE Buffer, Tris-Acetate-EDTA, 50X Solution, Electrophoresis, Fisher BioReagents™
Tris-Acetate-EDTA, CAS Number-77-86-1, 60-00-4, 6850-28-8, TAE, 4L, Gray, Tris (24%), Acetic Acid (5.0%), and EDTA (<2%)., DNase free, Pass Test, Filtered through a 0.2-micron filter., Electrophoresis, 50X Solution, Poly CUBE, Liquid, Protease free, DNase-, RNase- and Protease-Free, RT
TBE Buffer, Tris-Borate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents™
CAS: 10043-35-3 Synonym: TBE
Tris-Borate-Edta Buffer, for Electrophoresis, (10X Concentration), Fisher Chemical™
CAS: 610769-35-2 Molecular Formula: C14H30BN3O14 Molecular Weight (g/mol): 475.211 MDL Number: 4679 InChI Key: OSBLTNPMIGYQGY-UHFFFAOYSA-N PubChem CID: 21257725 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;boric acid SMILES: B(O)(O)O.C(CN(CC(=O)O)CC(=O)O)N(CC(=O)O)CC(=O)O.C(C(CO)(CO)N)O
Invitrogen™ 20X Bolt™ MES SDS Running Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats
Thermo Scientific™ Pierce™ Concentrated Buffer Stocks (10X and 20X)
Save time using Pierce Concentrated Buffer stock solutions (10X and 20X) with simple dilutions to expedite your experiment without sacrificing quality or consistency.
Thermo Scientific Chemicals TRIS-glycine native sample buffer (4X), pH 8.6
For preparation of protein samples for native gel electrophoresis
Tris, MP Biomedicals™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Trometamol, Tromethamine, Tris-[hydroxymethyl]amino-methane PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
Invitrogen™ TAE (10X), RNase-free
Molecular biology grade, Invitrogen 10X TAE is supplied in a 1 L bottle. The buffer is certified RNase-free, economical, and ready-to-use.
MP Biomedicals™ Tricine
Tricine is for use in electrophoresis buffers.
Invitrogen™ 10X TBE (powder)
Invitrogen 10X TBE (Tris-Borate-EDTA Buffer) is thoroughly tested for RNase and DNase contamination, making it ideal for use in sensitive application.
Tris, MP Biomedicals™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trometamol, Tromethamine, Tris-[hydroxymethyl]aminomethane, 2-Amino-2-(hydroxymethyl)-1,3-propanediol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
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