Protein Modification Reagents

Thermo Scientific™ Urea

Thermo Scientific™ Urea

Pure, crystalline urea (CH4N2O), CAS 57-13-6; denaturant with low UV-absorptivity for protein biology methods (elution, solubilization, refolding, storage).

Thermo Scientific™ 8M Guanidine-HCl Solution

Thermo Scientific™ 8M Guanidine-HCl Solution

Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.

Thermo Scientific™ Pierce™ TCEP-HCl, No-Weigh™ Format

Thermo Scientific™ Pierce™ TCEP-HCl, No-Weigh™ Format

Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).

Thermo Scientific™ Pierce™ SATA (N-succinimidyl S-acetylthioacetate)

Thermo Scientific™ Pierce™ SATA (N-succinimidyl S-acetylthioacetate)

Short (2.8A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies.

Thermo Scientific™ Bond-Breaker™ TCEP Solution, Neutral pH

Thermo Scientific™ Bond-Breaker™ TCEP Solution, Neutral pH

Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.

Thermo Scientific™ MS(PEG)4 Methyl-PEG-NHS-Ester Reagent

Thermo Scientific™ MS(PEG)4 Methyl-PEG-NHS-Ester Reagent

Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units).

Thermo Scientific™ Pierce™ DTT (Dithiothreitol), No-Weigh™ Format

Thermo Scientific™ Pierce™ DTT (Dithiothreitol), No-Weigh™ Format

Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.

Thermo Scientific™ Immobilized Papain (Agarose Resin)

Thermo Scientific™ Immobilized Papain (Agarose Resin)

Generate and purify Fab fragments from antibodies with cysteine-endopeptidase papain immobilized onto beaded agarose resin.

Thermo Scientific™ Pierce™ 2-Mercaptoethanol

Thermo Scientific™ Pierce™ 2-Mercaptoethanol

Pure, liquid (equals 14M), beta-mercaptoethanol (bME, 2BME, 2-ME, b-mer), CAS 60-24-2; thiol reducing agent for cleaving protein disulfide bonds (cystines cysteines).

Thermo Scientific™ Pierce™ Sulfo-NHS-Acetate

Thermo Scientific™ Pierce™ Sulfo-NHS-Acetate

Amine-reactive (NHS ester) compound to permanently block primary amines (e.g., protein lysine side chains) to prevent interactions or later conjugation.

Thermo Scientific™ Pierce™ Immobilized TCEP Disulfide Reducing Gel

Thermo Scientific™ Pierce™ Immobilized TCEP Disulfide Reducing Gel

Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized.

Invitrogen™ Measure-IT™ Thiol Assay Kit

Invitrogen™ Measure-IT™ Thiol Assay Kit

Provides easy and accurate quantitation of thiol

Thermo Scientific™ DTNB (Ellman's Reagent) (5,5-dithio-bis-(2-nitrobenzoic acid)

Thermo Scientific™ DTNB (Ellman's Reagent) (5,5-dithio-bis-(2-nitrobenzoic acid)

Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).

Thermo Scientific™ Pierce™ Hydroxylamine-HCl

Thermo Scientific™ Pierce™ Hydroxylamine-HCl

NH2OH (CAS 7803-49-8) to cleave Asn-Gly peptide bonds or deprotect sulfhydryls following S-acetylthioacetate modification with SATA reagent.

Thermo Scientific™ Trypsin, TPCK Treated

Thermo Scientific™ Trypsin, TPCK Treated

Cleave the carboxyl-side of Arg and Lys residues with trypsin that isTPCK-treated to block chymotrypsin activity; its immobilized form allows enzyme removal after digestion.

Thermo Scientific™ Pierce™ Mercaptoethylamine-HCl

Thermo Scientific™ Pierce™ Mercaptoethylamine-HCl

Pure, crystalline 2-aminoethanethiol (2-MEA-HCl, also called cysteamine-HCl), CAS 156-57-0; selectively reduces antibody hinge-region disulfide bonds.

Thermo Scientific™ Pierce™ DTT (Dithiothreitol)

Thermo Scientific™ Pierce™ DTT (Dithiothreitol)

Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.

Merck Millipore Proteinase K

Merck Millipore Proteinase K

From Tritirachium album

Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry

Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry

Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.

Thermo Scientific™ Pierce™ Traut's Reagent (2-iminothiolane)

Thermo Scientific™ Pierce™ Traut's Reagent (2-iminothiolane)

2-Iminothiolane-HCl (CAS 4781-83-3); an amine-reactive thiolation reagent to cap protein primary amines with sulfhydryl groups for immediate crosslinking.

Thermo Scientific™ Pierce™ Iodoacetamide, Single-Use

Thermo Scientific™ Pierce™ Iodoacetamide, Single-Use

Thermo Scientific™ Pierce™ Iodoacetamide No-Weigh™ Format is a sulfhydryl-reactive alkylating reagent used to block reduced cysteine residues for protein characterization and peptide mapping.

Thermo Scientific™ Pierce™ Cysteine-HCl

Thermo Scientific™ Pierce™ Cysteine-HCl

Make sulfhydryl assay standards or perform protein refolding experiments using this pure, crystalline cysteine hydrochloride (Cys-HCl) CAS 52-89-1.

Thermo Scientific™ Immobilized Pepsin (Agarose Resin)

Thermo Scientific™ Immobilized Pepsin (Agarose Resin)

Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.

Thermo Scientific™ In-Solution Tryptic Digestion and Guanidination Kit

Thermo Scientific™ In-Solution Tryptic Digestion and Guanidination Kit

Alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry (MS) using proteomics-grade trypsin, buffers and reagents.

Thermo Scientific™ TMT10plex™ Isobaric Label Reagent Set, 8 x 0.2 mg

Thermo Scientific™ TMT10plex™ Isobaric Label Reagent Set, 8 x 0.2 mg

TMT™ Isobaric Mass Tagging Kits and Reagents enable multiplex quantitation of proteins extracted from cells and tissues using tandem mass spectrometry.

Thermo Scientific™ Pierce™ Sodium meta-Periodate

Thermo Scientific™ Pierce™ Sodium meta-Periodate

Gently oxidize glycoprotein carbohydrate sugars to create reactive aldehydes (carbonyls) for crosslinking and immobilization using NaIO4 (CAS 7790-28-5).

Thermo Scientific™ TMS(PEG)12 Branched Methyl-PEG-NHS-Ester Reagent

Thermo Scientific™ TMS(PEG)12 Branched Methyl-PEG-NHS-Ester Reagent

A three-branched, polyethylene glycol compound (3 times 12 PEG units) activated as an NHS ester for covalent PEGylation of primary amines on proteins or assay surfaces.

Thermo Scientific™ CT(PEG)12 Carboxy-PEG-Thiol Compound

Thermo Scientific™ CT(PEG)12 Carboxy-PEG-Thiol Compound

CT(PEG)n: carboxylic acid and sulfhydryl compound with a 12-unit polyethylene glycol (PEG) spacer; carboxylate and pegylate quantum dots, gold surfaces, silver.

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