Thermo Scientific™ Pierce™ TCEP-HCl, No-Weigh™ Format

Product Code.	Quantity	unitSize
15780329	10 x 1 mg	Pack of 10
10530434	1 g	1g
11856784	10 g	10g

Product Code. 15780329 Supplier Thermo Scientific™ Supplier No. A35349

Description

Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).

Thermo Scientific™ Pierce TCEP-HCl is pure, crystalline Tris(2-carboxyethyl)phosphine hydrochloride, a thiol-free compound that is highly effective at reducing protein and peptide disulfide bonds, as well as being odorless and stable.

Thermo Scientific No-Weigh products are specialty reagents provided in pre-aliquoted format. The pre-weighed packaging helps prevent loss of reagent reactivity and contamination over time by minimizing repetitive opening and closing of vial. The format enables use of fresh vial of reagent each time, eliminating hassle of weighing small amounts of reagents and reducing concerns over reagent stability.

• Odorless: Unlike DTT or BME, TCEP is odor-free and can reduce proteins conveniently on bench
• Specific: Selective and complete reduction of even most stable water-soluble disulfides over wide pH range
• Simple: Effective reduction at room temperature and pH 5 in less than five minutes
• Stable: Inherent stability and resistant to air oxidation eliminates need for any special precautions while handling or storing; non-volatile and non-reactive toward other functional groups found in proteins
• Efficient: For most applications, 5 to 50mM TCEP provides sufficient molar excess to effectively reduce peptide or protein disulfide bonds within few minutes at room temperature
• Compatible: With TCEP, removal of reducing agent is not necessary prior to most applications, (e.g. histidine-tagged protein purification, maleimide conjugations)

Specifications

• Content And Storage: Store at room temperature in a sealed container to prevent oxidation.
• Shipping Condition: Room Temperature
• Product Line: No-Weigh, Pierce
• Form: Solid
• Quantity: 10 x 1 mg

Frequently Asked Questions (FAQs)

Are other TCEP-based products available besides Immobilized TCEP Disulfide Reducing Gel?

Yes. We offer TCEP-HCl (1g solid, Cat. No. 20490, 10g solid, Cat. No. 20491), TCEP-HCl 10 x 1 mg No-weigh format, Cat. No. A35349 and TCEP Solution, Neutral pH (5 mL of stable 500 mM TCEP, Cat. No. 77720).

Can other reducing agents other than DTT or BME be used to reduce proteins prior to electrophoresis? For example, what about TCEP (Tris Carboxy Ethyl Phosphene)?

TCEP, Tris Carboxy Ethyl Phosphene is an alternative sulfhydryl reducing agent for protein samples. It is an extremely potent and effective reducing agent for particularly ‘difficult' proteins. It is compatible with the Tris-Glycine gels and NuPAGE gels. It should be added to the sample buffer for these systems. 20 mM final (maximum) concentration is sufficient for samples. You may add alkylating agents, e.g. Iodine (50 mM Iodoacetic acid), to prevent re-forming of S-S bonds but it is not necessary. Do not heat because this will hydrolyze much of your sample. Instead let the sample sit for several minutes at RT and then load.

For Research Use Only. Not for use in diagnostic procedures.