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Thermo Scientific™ Pierce™ Iodoacetic Acid

Get rapid carboxymethylation of reduced cysteine residues with Thermo Scientific™ Pierce™ Iodoacetic Acid and Iodoacetamide.

Brand:  Thermo Scientific™ 35603

Code : PN

Additional Details : CAS Number : 108-20-3 Weight : 1.81440kg

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Product Code. 11899300

  • £74.50 / 500mg


Estimated Shipment
13-10-2017
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Description

Description

Iodoacetic Acid (IAA) can react with several protein functional groups but is typically used for specific S-carboxymethylation of sulfhydryls (reduced cysteines).

Iodoacetic acid reacts with sulfhydryls on cysteines, imidazolyl side chain nitrogens of histidines, the thioether of methionine and the primary amine group of lysines.The rate of reaction and specificity is dependent on the ionization level, which can be manipulated by the pH of the reaction condition.

Highlights:
  • React at slightly alkaline pH for specific S-carboxymethylation of free sulfhydryls
  • React at low pH for specific carboxymethylation of methionines
  • React at high pH to favor carboxymethylation of histidines and lysines
  • Methylate reduced cysteine peptide fragments in protease digests for mass spectrometry
Specifications

Specifications

Carboxymethane
Iodoacetic Acid
185.95
27mL of 100mM solution
Sulfhydryl
Iodoacetic acid
500mg
No