Thermo Scientific™ Pierce™ Iodoacetic Acid

Get rapid carboxymethylation of reduced cysteine residues with Thermo Scientific™ Pierce™ Iodoacetic Acid and Iodoacetamide.

Brand: Thermo Scientific™

Manufacturer Part Number: 35603

UNSPSC: 12161502

Code: PN

Additional Details:
Additional Details: CAS Number: 108-20-3 Weight: 1.81440kg

Product Code. 11899300

Quantity Price
1 £ 74.5 / 500mg
EU Stock 5
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Description and Specification


Formulation Iodoacetic acid
Reversible No
Molecular Weight 185.95
Sufficient For 27mL of 100mM solution
Description Iodoacetic Acid
Chemical Reactivity Sulfhydryl
Capping Group Carboxymethane
Quantity 500mg

Iodoacetic Acid (IAA) can react with several protein functional groups but is typically used for specific S-carboxymethylation of sulfhydryls (reduced cysteines).

Iodoacetic acid reacts with sulfhydryls on cysteines, imidazolyl side chain nitrogens of histidines, the thioether of methionine and the primary amine group of lysines.The rate of reaction and specificity is dependent on the ionization level, which can be manipulated by the pH of the reaction condition.

  • React at slightly alkaline pH for specific S-carboxymethylation of free sulfhydryls
  • React at low pH for specific carboxymethylation of methionines
  • React at high pH to favor carboxymethylation of histidines and lysines
  • Methylate reduced cysteine peptide fragments in protease digests for mass spectrometry