Crosslinking Reagents

Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format

Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format

Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.

Thermo Scientific™ BS3 (bis(sulfosuccinimidyl)suberate)

Thermo Scientific™ BS3 (bis(sulfosuccinimidyl)suberate)

Crosslink amines to amines with BS3; composed of sulfo-NHS-ester groups separated by an 11.4A, water-soluble spacer arm that is useful for cell-surface protein crosslinking.

Thermo Scientific™ Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format

Thermo Scientific™ Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format

Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.

Thermo Scientific™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format

Thermo Scientific™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format

Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.

Thermo Scientific™ DSS (disuccinimidyl suberate), No-Weigh™ Format

Thermo Scientific™ DSS (disuccinimidyl suberate), No-Weigh™ Format

Crosslink amines to amines with disuccinimidyl suberate composed of NHS-ester groups with a membrane-permeable, mid-length spacer for intracellular protein crosslinking.

Thermo Scientific™ BMOE (bismaleimidoethane)

Thermo Scientific™ BMOE (bismaleimidoethane)

Crosslink sulfhydryls to sulfhydryls (cysteine residues in proteins or peptides) with this short crosslinker composed of maleimide groups and a 7-atom (8.0A) spacer arm.

Thermo Scientific™ BMB (1,4-bismaleimidobutane)

Thermo Scientific™ BMB (1,4-bismaleimidobutane)

Crosslink sulfhydryls to sulfhydryls (cysteine residues in proteins or peptides) with this mid-length crosslinker composed of maleimide groups and 9-atom (10.9A) spacer arm.

Thermo Scientific™ BMPS (N-β-maleimidopropyl-oxysuccinimide ester)

Thermo Scientific™ BMPS (N-β-maleimidopropyl-oxysuccinimide ester)

Crosslink amines to sulfhydryls with this very short amine-to-sulfhydryl crosslinker composed of NHS-ester and maleimide groups at ends of an aliphatic, 5-atom spacer arm.

Thermo Scientific™ Pierce™ DSP, No-Weigh™ Format

Thermo Scientific™ Pierce™ DSP, No-Weigh™ Format

Crosslink amines to amines with dithiobis succinimidyl propionate, composed of NHS-ester groups and disulfide-containing, 12.0A spacer arm and cleavable with reducing agents.

Thermo Scientific™ EGS (ethylene glycol bis(succinimidyl succinate))

Thermo Scientific™ EGS (ethylene glycol bis(succinimidyl succinate))

Crosslink amines to amines with this membrane permeable crosslinker composed of NHS-ester groups and a 16.1A hydroxylamine-cleavable spacer arm.

Thermo Scientific™ NHS (N-hydroxysuccinimide)

Thermo Scientific™ NHS (N-hydroxysuccinimide)

Prepare amine-reactive NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysuccinimide, CAS 6066-82-6.

Thermo Scientific™ EMCS (N-ε-malemidocaproyl-oxysuccinimide ester)

Thermo Scientific™ EMCS (N-ε-malemidocaproyl-oxysuccinimide ester)

Crosslink amines to sulfhydryls with this mid-length crosslinker composed of NHS-ester and maleimide groups at ends of an aliphatic, 8-atom (9.4A) spacer arm.

Thermo Scientific™ SM(PEG)2 (PEGylated SMCC crosslinker)

Thermo Scientific™ SM(PEG)2 (PEGylated SMCC crosslinker)

Crosslink amines to sulfhydryls with these crosslinkers containing NHS-ester and maleimide groups at ends of water-soluble polyethylene glycol spacer arms (17.6 to 95.2A).

Thermo Scientific™ SM(PEG)8 (PEGylated, long-chain SMCC crosslinker)

Thermo Scientific™ SM(PEG)8 (PEGylated, long-chain SMCC crosslinker)

Crosslink amines to sulfhydryls with these crosslinkers containing NHS-ester and maleimide groups at ends of water-soluble polyethylene glycol spacer arms (17.6 to 95.2A).

Thermo Scientific™ DTBP (Wang and Richard's Reagent)

Thermo Scientific™ DTBP (Wang and Richard's Reagent)

Crosslink amines to amines with dithiobispropionimidate; composed of imidoester groups at either end of a reducible (cleavable) disulfide-containing spacer arm.

Thermo Scientific™ DFDNB (1,5-difluoro-2,4-dinitrobenzene)

Thermo Scientific™ DFDNB (1,5-difluoro-2,4-dinitrobenzene)

Crosslink amines to amines via stable arylamine bonds using 1,5-difluoro-2,4-dinitrobenzene; an aryl halide compound with a short spacer arm (3.0A) and fluorine atoms.

Thermo Scientific™ LC-SMCC (succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxy-(6-amidocaproate))

Thermo Scientific™ LC-SMCC (succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxy-(6-amidocaproate))

Crosslink amines to sulfhydryls with this long crosslinker composed of NHS-ester and maleimide groups at ends of a stabilizing cyclohexane-containing, 15-atom spacer arm.

Thermo Scientific™ Pierce™ Premium Grade EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)

Thermo Scientific™ Pierce™ Premium Grade EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)

Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this popular carbodiimide crosslinker.

Thermo Scientific™ DMP (dimethyl pimelimidate)

Thermo Scientific™ DMP (dimethyl pimelimidate)

Crosslink amines to amines with dimethyl pimelimidate (CAS 58537-94-3); composed of imidoester groups and a membrane-permeable, 7-atom spacer arm.

Thermo Scientific™ SM(PEG)4 (PEGylated SMCC crosslinker)

Thermo Scientific™ SM(PEG)4 (PEGylated SMCC crosslinker)

Crosslink amines to sulfhydryls with these crosslinkers containing NHS-ester and maleimide groups at ends of water-soluble polyethylene glycol spacer arms (17.6 to 95.2A).

Thermo Scientific™ Sulfo-KMUS (N-κ-maleimidoundecanoyl-oxysulfosuccinimide ester)

Thermo Scientific™ Sulfo-KMUS (N-κ-maleimidoundecanoyl-oxysulfosuccinimide ester)

Crosslink amines to sulfhydryls with this long crosslinker composed of sulfo-NHS-ester and maleimide groups at ends of an aliphatic, 13-atom (16.3A) spacer arm.

Thermo Scientific™ DTSSP (3,3'-dithiobis(sulfosuccinimidyl propionate))

Thermo Scientific™ DTSSP (3,3'-dithiobis(sulfosuccinimidyl propionate))

Crosslink amines to amines with Sulfo-DSP; composed of sulfo-NHS-ester groups and disulfide spacer arm (12.0A) that is cleavable with reducing agents.

Thermo Scientific™ BMH (bismaleimidohexane)

Thermo Scientific™ BMH (bismaleimidohexane)

Crosslink sulfhydryls to sulfhydryls (cysteine residues in proteins or peptides) with this long crosslinker composed of maleimide groups and 11-atom (13.0A) spacer arm.

Thermo Scientific™ PEG12-SPDP (PEGylated, long-chain SPDP crosslinker)

Thermo Scientific™ PEG12-SPDP (PEGylated, long-chain SPDP crosslinker)

Very long (54.1A) amine-to-sulfhydryl crosslinker; NHS-ester and pyridyldithiol groups at ends of 12-unit polyethylene glycol spacer arm; forms cleavable disulfide bonds; water-soluble.

Thermo Scientific™ Pierce™ Premium Grade SPDP

Thermo Scientific™ Pierce™ Premium Grade SPDP

Crosslink proteins and other biomolecules in applications where product integrity and risk minimization are paramount with Thermo Scientific™ Pierce™ Premium Grade SPDP, a high-quality formulation of this popular pyridyldithiol-based amine-to-sulfhydryl crosslinker.

Thermo Scientific™ Sulfo-SBED Biotin Label Transfer Reagent, No-Weigh™ Format

Thermo Scientific™ Sulfo-SBED Biotin Label Transfer Reagent, No-Weigh™ Format

Chemically tag purified proteins at primary amines to covalently crosslink and transfer biotinylation to protein interaction binding partners.

Thermo Scientific™ SPDP (succinimidyl 3-(2-pyridyldithio)propionate)

Thermo Scientific™ SPDP (succinimidyl 3-(2-pyridyldithio)propionate)

Crosslink amines to sulfhydryls with this crosslinker composed of NHS-ester and pyridyldithiol groups on 3-atom spacer arm; forms cleavable disulfide bonds with cysteines.

Thermo Scientific™ GMBS (N-γ-maleimidobutyryl-oxysuccinimide ester)

Thermo Scientific™ GMBS (N-γ-maleimidobutyryl-oxysuccinimide ester)

Crosslink amines to sulfhydryls with this short crosslinker composed of NHS-ester and maleimide groups at ends of an aliphatic, 6-atom (7.3A) spacer arm.

  spinner