Thermo Scientific Pierce™ Pierce™ SM(PEG)12, No-Weigh™ Format

Product Code.	Quantity	unitSize
15981130	10 x 1 mg	Pack of 10

Product Code. 15981130 Supplier Thermo Scientific Pierce™ Supplier No. A35398

Description

Thermo Scientific™ Pierce™ SM(PEG)12 is hetero-bifunctional crosslinker with N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules.

Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging helps prevent loss of reagent reactivity and contamination over time by minimizing repetitive opening and closing of vial. The format enables use of fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.

• Reactive groups: NHS ester and maleimide
• Reactive toward: amino and sulfhydryl groups
• Irreversibly crosslink proteins or peptides by flexible PEG spacer arms
• Polyethylene glycol spacer arms help maintain conjugate solubility
• Pure compound with defined structure and molecular weight, ensuring reproducible protein-modification effects
• PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
• Ideal for performing controlled, two-step protein conjugations

Specifications

• Chemical Reactivity: Amine-Sulfhydryl
• Cleavable: No
• Description: BS(PEG)5
• Molecular Weight (g/mol): 532.5
• PEGylated: Yes
• Spacer Arm Length: 53.4 Å
• Content And Storage: Upon receipt store desiccated at -20°C.
• Cell Permeability: No
• Shipping Condition: Wet Ice
• Product Line: No-Weigh, Pierce
• Labeling Method: Chemical Labeling
• Crosslinker Type: Heterobifunctional
• Reactive Moiety: Maleimide, NHS Ester
• Spacer: Long (>30 Å)
• Form: Solid
• Quantity: 10 x 1 mg
• Solubility: Water
• Format: Standard, Single-use
• Water Soluble: Yes
• Product Type: Crosslinker

Frequently Asked Questions (FAQs)

Can you provide the shelf-life for SM(PEG)12 (PEGylated, long-chain SMCC crosslinker)?

SM(PEG)12 (PEGylated, long-chain SMCC crosslinker) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Is there any advantage of choosing a Sulfo-NHS biotin with a longer spacer arm than EZ-Link Sulfo NHS Biotin?

Sometimes. The biotin has to insert 9 angstrom into the pocket of avidin, streptavidin or NeutrAvidin to bind. If steric hindrance may be a problem, using a biotinylation reagent with a longer spacer arm (Sulfo-NHS-LC Biotin or Sulfo-NHS-LC-LC Biotin) may result in greater purification or deteciton.

Why would I chose a Sulfo-NHS Biotin over its non-sulfonated counterpart?

The sodium sulfonate group lends water solubility to the reagent.
Note: The actual biotin moiety that is added is identical between NHS and Sulfo-NHS biotins that vary only in the addition of the sodium sulfonate on the leaving group. Researchers preferring a more water soluble biotin be added to their molecule should consider a PEGylated version of biotin.

For Research Use Only. Not for use in diagnostic procedures.