Thermo Scientific™ Pierce™ NHS-Azide Staudinger Ligation Reagents

Activated azido compounds to derivatize proteins or amine surfaces for selective conjugation, detection or purification by phosphine-modified probes.

Brand: Thermo Scientific™

Manufacturer Part Number: 26130

UNSPSC: 12161502

Code: PN

Additional Details:
Additional Details: Weight: 0.11340kg

Product Code. 11894534

Quantity Price
1 £ 236.0 / 100mg
EU Stock 4
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Description and Specification


Formulation Tetraethyleneglycol functionalized with succinimidyl and azido ends; liquid
Cleavable No
Sufficient For 257mL of typical 1X (1mM) reaction solution, or approx. 50 uses at 2mg reagent per use
Molecular Weight 388.37
Spacer Arm Length 18.9
Membrane Permeable Yes
Description Pierce NHS-PEG4-Azide
Chemical Reactivity Amine-to-Nonselective
PEGylated Yes
Water Soluble No
Quantity 100mg

These NHS-ester compounds react to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The azide (N3) group reacts with phosphine-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. In combination with different phosphine-activated compounds, these NHS-Azide reagents facilitate a variety of labeling and crosslinking experimental strategies for investigating protein interactions and cellular pathways.


  • Soluble – reagents easily dissolve in water-miscible solvents for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples

  • Compatible – reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents

  • Specific – NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)


  • Chemoselective – azide and phosphine groups do not react or interfere with components of biological samples but conjugate to one another with high efficiency

  • Pegylated – choose the very short NHS-Azide or one of the two varieties whose spacer arms contain medium (4-unit) or long (12-unit) polyethylene glycol (PEG) groups for increased solubility and reach