Chromatography Media
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Filtered Search Results
Cytiva HiTrap™ TALON™ Crude Prepacked Columns
For purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). Cytiva Lifescience™ HiTrap™ TALON™ Crude Prepacked Columns offer a cobalt-based medium allowing protein purification under native or denaturing conditions.
| Particle Size | 60 to 160 μm |
|---|---|
| Ligand Type | Talon Superflow Cobalt-IMAC |
| Flow Rate | 1 to 4 mL/min |
| Matrix | 6% Cross-Linked Agarose |
| Storage Buffer | 20% Ethanol |
| pH Range | 3 to 12 |
| Product Type | HiTrap Talon crude prepacked Column |
| Material | Polypropylene |
| Storage Requirements | 4°C to 8°C |
| For Use With (Application) | For PurifiCatIon of Histidine-Tagged Recombinant Proteins by Immobilized Metal Affinity Chromatography, Used Buffers and Easily Regenerated with 0.5 M NaOH |
| Max. Pressure | 3 bar |
Cytiva Capto™ DEAE Chromatography Media
Used for fast, efficient capture and intermediate protein purification
Cytiva NHS-Activated Sepharose™ 4 Fast Flow Affinity Media
Use for coupling amino-containing smaller proteins and peptides. Cytiva Life Sciences™ NHS-Activated Sepharose™ 4 Fast Flow Affinity Media yields coupled products that are very stable, especially at high pH.
Cytiva Strep-Tactin™ XT Sepharose™ Chromatography Resin
Chromatography resin for purification of recombinant proteins tagged with a Strep-tag™ II and Twin-Strep-tag™.
| Particle Size | 34 μm |
|---|---|
| Ligand Type | Strep-Tactin XT |
| Density (Metric) | Approx. 5 mg/mL resin |
| Matrix | Rigid, cross-linked agarose, spherical |
| Stability | Stable in all commonly used aqueous buffers |
| pH Range | 6 to 10 pH |
| Binding Capacity | Approx. 10 mg protein with Strep-tag II or Twin-Strep-tag/mL of resin |
| Storage Requirements | 2 to 8°C in 20% ethanol |
| For Use With (Application) | Purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag |
Cytiva Sephaorose™ Fast Flow Anion Exchange Media: Q
Use for preparative protein separations in both research and industrial applications. Cytiva Q Sepharose™ Fast Flow Chromatography Media is a strong anion exchanger based on the well established Sepharose Fast Flow ion exchange platform.
Cytiva Phenyl Sepharose™ 6 Fast Flow Chromatography Media
Used for capture and intermediate purification requiring low to medium hydrophobicity
| Ligand Type | Phenyl |
|---|---|
| Matrix | 6% Cross-Linked Agarose |
| pH Range | 3 to 13 |
| For Use With (Application) | For Capture and Intermediate PurifiCatIon Requiring Low to Medium Hydrophobicity |
Cytiva HiTrap™ Capto™ PlasmidSelect
For the purification of supercoiled DNA to high quality for gene therapy and DNA vaccine applications
| Matrix | Capto™ PlasmidSelect |
|---|---|
| Length (Metric) | 2.5 cm |
| Product Type | Chromatography Resin |
| For Use With (Application) | Purification of Supercoiled DNA From Research Scale to cGMP Production |
| Max. Pressure | 5 bar (0.5 MPa) |
Cytiva IMAC Sepharose™ 6 Fast Flow Affinity Media
Used for purifying proteins and peptides that have an affinity for metal ions. Cytiva IMAC Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix.
| Product Type | IMAC Sepharose 6 Fast Flow Media |
|---|---|
| For Use With (Application) | For Purifying Proteins and Peptides That Have an Affinity for Metal Ions |
Cytiva Sepharose™ Fast Flow Cation Exchange Media
SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification. CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.
| Particle Size | 45 to 165 μm |
|---|---|
| pH Range | 4 to 13 |
Cytiva Butyl Sepharose™ 4 Fast Flow Chromatography Media
Used for capture and intermediate purification of larger proteins
| Matrix | Based on cross-linked 4% agarose |
|---|---|
| Product Type | Butyl Sepharose 4 Fast Flow Media |
Cytiva DEAE Sepharose™ Fast Flow Chromatography Media
For preparative protein separations in both research and industrial applications. Cytiva Life Sciences™ DEAE Sepharose™ Fast Flow Chromatography Media can be used for capture and intermediate purification of wide range of approved biopharmaceuticals.
Cytiva Sephaorose™ Fast Flow Anion Exchange Media: Q
Use for preparative protein separations in both research and industrial applications. Cytiva Q Sepharose™ Fast Flow Chromatography Media is a strong anion exchanger based on the well established Sepharose Fast Flow ion exchange platform.
Cytiva TALON™ Superflow Medium
Offers different selectivity compared to nickel-charged media. Cytiva TALON™ Superflow Medium used for purification of histidine-tagged recombinant proteins by IMAC.
| Particle Size | 60 to 160 μm |
|---|---|
| Ligand Type | Talon Superflow Cobalt-IMAC |
| Matrix | 6% Cross-Linked Agarose |
| Storage Buffer | 20% Ethanol |
| pH Range | 3 to 12 |
| Product Type | Talon Superflow Media |
| Binding Capacity | Up to 20 mg Histidine-tagged Protein |
| Storage Requirements | 4°C to 8°C |
| For Use With (Application) | For PurifiCatIon of Histidine-Tagged Recombinant Pby Immobilized Metal Affinity Chromatography |
Cytiva Blue Sepharose™ 6 Fast Flow Chromatography Media
Blue dye binds to proteins such as albumin and lipoproteins as well as enzymes like kinases and dehydrogenases
Cytiva Phenyl Sepharose™ High Performance Media
Designed for intermediate and polishing step purification steps