Cytiva Blue Sepharose™ 6 Fast Flow Chromatography Media

Product Code. 10271350

Brand: Cytiva 17094801

Description

Blue dye binds to proteins such as albumin and lipoproteins as well as enzymes like kinases and dehydrogenases

This medium is well established as adsorbent for albumin and interferon at laboratory and process scale.

• Cibacron Blue 3G is covalently coupled to the base matrix allowing for low ligand leakage and high capacity
• The blue dye binds proteins, such as albumin, interferon, lipoproteins and blood coagulation factors
• The dye also binds enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+
• Avg. particle size: 90μm
• Volume: 50mL

Developed and tested in co-operation with large-scale manufacturers and is used in routine commercial production.

• For large-scale purification of interferon and albumin
• For isolating groups of enzymes and removing of albumin
• For industrial applications and other approved applications

Certifications

Certificate of Analysis

Specifications

• Quantity: 50 mL
• Stability: Stable in commonly used aqueous Buffers: 70% Ethanol, 6 M Guanidine Hydrochloric, 8 M Urea
• For Use With (Application): For Albumin and Interferon at Both Laboratory and Process Scale
• Storage Requirements: 2°C to 8°C
• Product Type: Blue Sepharose 6 Fast Flow Media
• Flow Rate: 250 to 400 cm/Hoμr
• Particle Size: 45 to 165 μm
• Height (English) Bed: 9.84 in.
• Height (Metric) Bed: 25 cm
• Includes: Certificate of Analysis
• Max. Pressure: 100 kPa
• Matrix: 6% Cross-Linked Agarose
• pH Range: 4 to 12
• Storage Buffer: 20% Ethanol, 0.1 M Potassium Phosphate Buffer
• Ligand Type: Cibacron Blue

Safety and Handling

Product Identifier

• Blue Sepharose 6 Fast Flow, 50 ml

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol
• UNKNOWN ACUTE-1.2 per cent of the mixture consists of component(s) of unknown acute toxicity.
• UNKNOWN AQUATIC-Contains 16.5% of components with unknown hazards to the aquatic environment.