Cytiva NHS-Activated Sepharose™ 4 Fast Flow Affinity Media

Product Code.	Quantity	unitSize
10343240	25 mL	25mL

Product Code. 10343240

Brand: Cytiva 17090601

Description

Use for coupling amino-containing smaller proteins and peptides. Cytiva Life Sciences™ NHS-Activated Sepharose™ 4 Fast Flow Affinity Media yields coupled products that are very stable, especially at high pH.

• Composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides
• NHS (N-hydroxysuccinimide) coupling forms chemically stable amide bond with ligands containing primary amino groups
• Resin provides spacer arm to coupled ligand
• Fast Flow matrix gives high productivity
• Coupled product is very stable, especially at high pH
• Available in range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development

Specifications

• Quantity: 25 mL
• Stability: Stable in commonly used aqueous Buffers provided that the ligand withstand the pH or the chemical environment
• For Use With (Application): For Coupling Amino-Containing Smaller Proteins and Peptides
• Storage Requirements: 2°C to 8°C
• Product Type: NHS-Activated Sepharose 4 Fast Flow Media
• Flow Rate: 150 to 250 cm/Hoμr
• Particle Size: 45 to 165 μm
• Height (English) Bed: 9.84 in.
• Height (Metric) Bed: 25 cm
• Max. Pressure: 100 kPa
• Matrix: 4% Cross-Linked Agarose
• pH Range: 3 to 13
• Storage Buffer: 100% Isopropanol
• Ligand Type: N-hydroxysuccinimide (NHS) Activated

Safety and Handling

Signal Word

• Danger

Hazard Category

• Flammable liquid Category 2
• Serious eye damage/eye irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H225-Highly flammable liquid and vapour.
• H319-Causes serious eye irritation.
• H336-May cause drowsiness or dizziness.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : propan-2-ol