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Gel Filtration Media and Columns

A variety of columns and media for the size-based separation and purification of proteins, lipoproteins, peptides, etc. using gel filtration chromatography techniques.
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1 – 15 of 49 results
1

Cytiva Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

EDGE
2

Cytiva HiTrap™ Heparin HP Columns

Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.

EDGE
Particle Size 34 μm
Ligand Type 10mg Heparin/mL medium
Height (Metric) 2.5cm
Content And Storage 4° to 30°C
pH Range 5 to 10
Product Type Heparin HP Column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Material Polypropylene
For Use With (Application) Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
3

Cytiva Sephadex™ G-25 Media

Use of Sephadex™ is well established as a gel filtration medium for desalting and buffer exchange in industrial applications. Cytiva Life Sciences™ Sephadex™ G-25 Media quickly desalts, removes contaminants and transfers to a new buffer in a single step.

EDGE
Height (Metric) 30cm
Content And Storage 4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH
Bed Volume 4 to 6mL/g in Distilled Water
Format Solid Spherical Bead
pH Range 2 to 13
Product Type Sephadex G-25 Media
Diameter (Metric) Inner 2.6cm
For Use With (Application) Desalting and Buffer exchange and removal free label in industrial applications
Support Type Cross-linked Dextran
Height (English) 11.81 in.
4

Cytiva Gel Filtration Calibration Kits

Calibrate gel filtration columns simply, reliably. Cytiva Life Sciences™ Gel Filtration Calibration Kits contain a well-defined series of protein standards.

Content And Storage 2° to 8°C
Product Type Calibration Kit
For Use With (Equipment) High-resolution media and prepacked column
Quantity Each
For Use With (Application) Calibration of high-resolution gel filtration media and column such as Superdex™, Superose and Sephacryl
5

Cytiva Sephadex™ LH-20

Use for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Cytiva Life Sciences™Sephadex™ LH-20 is a liquid chromatography media useful to analytical and industrial scale for preparation of closely related molecular species.

Particle Size 18 to 111 μm in dry form
Height (Metric) 30cm
Flow Rate 720 cm/hr.
Content And Storage 4 to 30°C
Format Solid Spherical Bead
pH Range 2 to 13
Product Type Sephadex LH-20 Media
Sample Type Steroid, Terpenoid, Lipid, low molecular weight Peptide
Diameter (Metric) Inner 2.6cm
For Use With (Application) For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures
Support Type Hydroxypropylated, cross-linked Dextran
Max. Pressure 25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.
Height (English) 11.81 in.
6

Cytiva HiTrap™ NHS-Activated HP Columns

Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.

Particle Size 34 μm
Ligand Type 10μM NHS groups/mL medium
Height (Metric) 2.5cm
Content And Storage 4° to 8°C
pH Range 3 to 12
Product Type NHS-Activated HP Column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Material Polypropylene
For Use With (Application) Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
7

Cytiva Sephacryl™ S-300 High Resolution Media

Offer good recovery and low nonspecific adsorption. Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules. Enable gel filtration at laboratory and industrial scale, and may be purchased in pre-packed columns.

8

Cytiva Sephadex™ G-25 DNA Grade SF Cleanup Gel

Tested to ensure reproducibly high recovery of DNA (10 bases in length) from small molecules by gel filtration

9

Cytiva Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

10

Cytiva Superdex™ 200 Prep Grade

Produce separation medium with high selectivity and resolution. Cytiva Life Sciences™ Superdex™ 200 Prep Grade for purifying proteins of fractionation range: 10000 to 600000D

11

Cytiva Sepharose Agarose Gel Filtration Media

Choose from a wide range of bead-formed agarose-based gel filtration media. Cytiva Life Sciences™ Sepharose Agarose Gel Filtration Media shows good separation over a wide molecular weight range.

Format Solid Bead
Product Type Sepharose Agarose Gel Filtration Media
Quantity 1 L
For Use With (Application) Allow characterization or cleaning up of samples containing components of diverse molecular weight
12

Cytiva Sephadex™ G50 Medium Media

Suitable for gel filtration

Particle Size 100 to 300 μm in wet form, 50 to 150 μm in dry form
Height (Metric) 10cm
Bed Volume 9 to 11mg/g
Format Solid Bead
pH Range 2 to 10
Product Type Sephadex G50 Media
Sample Type DNA
Diameter (Metric) Inner 5cm
Support Type Cross-linked Dextran
Height (English) 3.93 in.
13

Cytiva Superdex 30 Increase Precision 3.2/300 GL Prepacked SEC Column

Designed for high resolution in small-scale preparative purification and analysis for sample volumes up to 500μL

14

Cytiva HiTrap™ Capto™ Core 400

Designed for intermediate purification and polishing of viruses and other large biomolecules

15

Cytiva Sephadex™ G15 Media

Gel filtration medium for desalting and buffer exchange for small biomolecules greater than 1500 molecular weight