Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns

Product Code.	Flow Rate	Bed Volume	Diameter (Metric) Inner	unitSize
11330292	<0.5 mL/min.	120 mL	1.6cm	Each
11350292	<1.3 mL/min.	120 mL	1.6cm	Each
11360292	<0.5 mL/min.	320 mL	2.6cm	Each

Product Code. 11330292

Brand: Cytiva 28935604

Description

For reproducible protein purification, separating polysaccharides, macromolecules with extended structures and small plasmids

Aids in preparative separation of biomolecules from up to 5mL (for 16mm column I.D.) and 13mL (for 26mm column I.D.).

• Provides maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability
• Prepacked columns for use with AKTAdesign™ or other pumped chromatography systems
• Available in two different prepacked column sizes - 16 x 600mm (120mL) and 26 x 60mm (320mL)
• Maximum pressure (over packed bed during operation) is 1.5 bar (22psi)

Media    

• Average particle size: 50μm
• Matrix: Allyl dextran and N, N'-methylenebisacrylamide
• pH Stability working range: 3 to 11
• pH Stability cleaning range: 2 to 13
• Chemical stability: all commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1% SDS, 20% ethanol, 30% propanol, 30% acetonitrile (tested at 40°C for one week), 0.5 M NaOH (only for cleaning-in-place)
• Pressure/Flow specification: >125cm/hr., 100kPa, XK 50/30, bed height 15cm

Column

• Material (bed support): Polymethyl-Methacrylate

HiPrep 16/60 Sephacryl S-400 HR

• Media:
• Fractionation [Mr] globular proteins: 2 x 104^-8 x 106
• Exclusion limit [Mr] (globular proteins): ≈9 x 106 (9000kDa)
• Fractionation range [Mr] DNA exclusion limit:    271bp
• Fractionation [Mr] Dextrans: 1 x 104^-2 x 106

HiPrep 16/60 Sephacryl S-500 HR

• Media:
• Exclusion limit for Dextrans: 100 x 106
• Fractionation range [Mr] DNA exclusion limit: 1078bp
• Fractionation [Mr] Dextrans: 4 x 104^-2 x 107

Specifications

• Quantity: Each
• Flow Rate: <0.5 mL/min.
• Particle Size: 25 to 75 μm
• For Use With (Equipment): Low to medium pressure AKTA™ systems or pumped chromatography systems
• Bed Volume: 120 mL
• Product Type: Sephacryl HR Pre-packed Gel Filtration Column
• Diameter (Metric) Inner: 1.6cm
• For Use With (Application): Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
• Height (English): 23.62 in.
• Height (Metric): 60cm
• Material: Polypropylene
• Max. Pressure: 1.5 bar [0.15 MPa] (22 psi)
• pH Range: 3 to 11
• Support Type: Allyl Dextran and N,N'-methylenebisacrylamide
• Sample Type: Polysaccharides, macromolecules with extended structures and even small plasmids
• Content And Storage: 4 to 30°C, 20% Ethanol

Safety and Handling

Product Identifier

• HiPrep 16/60 Sephacryl S-400 HR

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol