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DNA Sequencing

Chemical reagents, kits, and other products used for DNA sequencing; includes sequencing and pre-sequencing kits, purification kits, buffers, standards, etc.
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1
Promotions Available

Applied Biosystems™ BigDye™ Terminator v1.1 Cycle Sequencing Kit

This BigDye™ Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules.

EDGE
Product Type Sequencing Buffer
For Use With (Equipment) SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐Well and 384‐Well Thermal Cyclers
Technique Fluorescent Dye Terminator Sequencing
Template Compatibility BAC DNA, Plasmid DNA (≤15 Kb), PCR Amplicons, Single Stranded DNA
Product Line BigDye™ Terminator
2
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Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit

The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

Format 12-well Strips,8-well Strip,96- or 384-well Plate
Product Type Sequencing Buffer
For Use With (Equipment) SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers
Technique Fluorescent Dye Terminator Sequencing
Product Line BigDye™ Terminator
3
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Thermo Scientific™ Jurkat Genomic DNA

Use this pure human genomic DNA as a control in DNA methylation analysis, genomic DNA library construction, PCR, and Southern blotting.

4
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Applied Biosystems™ Sanger Sequencing Kit

Sanger Sequencing Kit includes all reagents needed for sample preparation for sanger sequencing

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5

Applied Biosystems™ 3730xl DNA Analyzer 96-Capillary Array, 50 cm

This is a capillary array for the Applied Biosystems™ 3730XL DNA analyzer.

6

Applied Biosystems™ dGTP BigDye™ Terminator Ready Reaction Kit

Ideal for working with GT- and G-rich and other difficult-to-sequence templates

7
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Applied Biosystems™ BDT/POP/Buffer Bundle

The BDT/POP/Buffer Bundle includes a BigDye Terminator 3.1 Cycle Sequencing Kit, POP-7 Polymer, Anode Buffer Containers, and Cathode Buffer Containers for your 3500 or SeqStudio Flex genetic analyzer. All products are offered in a single, easy-to-order bundle to provide key reagents for Sanger sequencing workflows.

Format Kit
Product Type Sequencing Buffer
Enzyme Proprietary
Control Type Plasmid DNA
Sample Type Purified DNA
Isolation Technology Enzymatic, Magnetic Bead
Label or Dye Various
For Use With (Application) Standard Sequencing
Species Various
Sequencing Type Genome and DNA Sequencing
Detection Method Fluorescent Dye
8
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Invitrogen™ Collibri™ DNA Library Preparation Kit Modules

The Invitrogen Collibri DNA Library Preparation Kit Modules are designed for a custom build of next-generation sequencing (NGS) workflow on Illumina systems.

Format Kit
For Use With (Application) DNA Sequencing; Whole-Genome Sequencing, Amplicon Sequencing
Sequencing Type Genome and DNA Sequencing
Product Line Collibri
9

Applied Biosystems™ BigDye™ Terminator v1.1 & v3.1 5X Sequencing Buffer

This sequencing buffer is optimized for use with BigDye™ Terminator v1.1 and v3.1 Cycle Sequencing Kits.

EDGE
Shipping Condition Room Temperature
Buffer Sequencing Buffer
Content And Storage Store at 2°C to 8°C.
Form Liquid
Concentration 5X
For Use With (Application) Standard Sequencing
10

Applied Biosystems™ POP-7™ Polymer for 3730/3730xl DNA Analyzers

POP-7™ Polymer is a separation matrix for the 3730 and 3730xl DNA Analyzers. POP-7™ Polymer is customized for DNA sequencing and fragment analysis.

EDGE
Accuracy 98.5% up to 900 bases (for 50 cm capillary), 98.5% up to 700 bases (for 36 cm capillary)
Product Type Polymer
For Use With (Application) Standard Sequencing, Fragment Analysis (Denaturing)
Test Time 1 hr for 36 cm capillary (std. seq.), 2 hrs for 50 cm capillary (long read seq.)
Product Line POP-7
11
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Applied Biosystems™ Sequencing Standard, BigDye™ Terminator v3.1, for 3500/SeqStudio™ Flex

The DNA contained in the preparation has been lyophilized to maximize stability.The two hydrolytic enzymes used in this kit, shrimp alkaline phosphatase and exonuclease I, effectively remove excess dNTPs and primers.

EDGE
12
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TaqMan™ Ion Torrent™ Ion Library TaqMan™ Quantitation Kit

An easy-to-use, flexible solution for the accurate quantitation of library input for the Ion Torrent semiconductor sequencing workflow

EDGE
13
Promotions Available

Applied Biosystems™ POP-7™ Polymer For 3500 Dx Series

Conveniently offered in easy to use pouch packages

EDGE
Product Type POP-7™ Polymer
For Use With (Equipment) 3500 Dx Series
Size 1 Kit
14

Applied Biosystems™ GeneScan™ 1000 ROX™ dye Size Standard

The GeneScan™ 1000 ROX™ Size Standard is an internal lane size standard developed for use with Applied Biosystems™ fluorescence-based DNA electrophoresis systems.

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15

Applied Biosystems™ GeneScan™ 400HD ROX™ dye Size Standard

The GeneScan™ 400HD ROX™ Size Standard is a ROX™ dye-labeled size standard for the reproducible sizing of fragment analysis data. The standard contains 21 ROX™ dye-labeled DNA fragments.

EDGE
For Use With (Equipment) 3730xl DNA Analyzer, 3730 DNA Analyzer, 3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers, SeqStudio Genetic Analyzer
Label or Dye ROX
For Use With (Application) Fragment analysis
Product Line GeneScan, ROX