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Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit
Shop All Applied Biosystems ProductsDescription
The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.
- Improve the quality of your results for a wide range of sequencing applications
- Optimized for long read lengths
- Better dye mobility characteristics
- Improved performance reading through GT-rich regions
- Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
- Enhance your productivity and reduce costs
Get more uniform peak heights and improved accuracy
With BigDye™ Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.
Specifications
Specifications
| Content And Storage | • 1 x 800 μL tube of BigDye™ Terminator v3.1 Ready Reaction Mix • 1 tube M13 (-21) Primer • 1 tube pREF-BDT™ Control DNA • 2 x 1 mL tubes of 5X Sequencing Buffer (may be stored at 4°C) Store kit at -15°C to -25°C, and avoid repeated freeze-thaw cycles. |
| Format | 12-well Strips, 8-well Strip, 96- or 384-well Plate |
| For Use With (Equipment) | SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers |
| For Use With (Application) | AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), Mixed-Base Detection, Long-Read Sequencing, Resequencing, De Novo Sequencing - High Throughput (cDNA), GT-Rich or Difficult Template Sequencing |
| Product Line | BigDye™ Terminator |
| Product Type | Sequencing Buffer |
| Quantity | 100 Reactions |
| Technique | Fluorescent Dye Terminator Sequencing |
| Template Compatibility | BAC DNA, Lambda DNA, Genomic DNA (Bacterial), Single Stranded DNA, Rolling Circle Amplified Products, Fosmids, PCR Amplicons (50:50 Heterozygotes), Plasmid DNA (≤15 Kb), PCR Amplicons |
For Research Use Only. Not for use in diagnostic procedures.
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