Thermo Scientific™ T4 DNA Ligase, HC (30 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Thermo Scientific™ FastDigest NcoI
The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I).
Thermo Scientific™ RNase I (10 U/μL)
Ribonuclease I (RNase I) is an endoribonuclease that hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates.
Thermo Scientific™ FastDigest SacI
The FastDigest SacI restriction enzyme recognizes GAGCT^C sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI).
Thermo Scientific™ FastDigest BglII
Cut at A^GATCT sites with FastDigest™ BglII restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ DNase I, RNase-free, HC (50 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Thermo Scientific™ SalI (10 U/μL)
The SalI restriction enzyme recognizes G^TCGAC sites and cuts best at 37°C in O buffer.
Thermo Scientific™ FastDigest AatII
Cut at GACGT^C sites with Thermo Scientific™ FastDigest™ AatII, which performs best at 37°C in 5-15 minutes using universal FastDigest™ Buffer (Isoschizomers: ZraI).
Thermo Scientific™ TaqI (10 U/μL)
The TaqI restriction enzyme recognizes T^CGA sites and cuts best at 65°C in its own unique buffer.
Thermo Scientific™ FastDigest BglI
Cut at GCCNNNN^NGGC sites with Thermo Scientific™ FastDigest™ BglI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ SacI (10 U/μL)
Cut at GAGCT^C sites with SacI restriction enzyme, which performs best at 37°C in its own unique buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI).
Thermo Scientific™ PfeI (TfiI) (10 U/μL)
Cut at G^AWTC sites with PfeI (TfiI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: TfiI).
Thermo Scientific™ XagI (EcoNI) (10 U/μL)
Cut at CCTNN^NNNAGG sites with XagI (EcoNI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: BstENI, EcoNI).
Thermo Scientific™ FastDigest Pfl23II
Cut at C^GTACG sites with Thermo Scientific™ FastDigest™ BsiWI (Pfl23II), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PspLI).
Thermo Scientific™ HinfI (10 U/μL)
The HinfI restriction enzyme recognizes G^ANTC sites and cuts best at 37°C in R buffer.