Thermo Scientific™ T4 DNA Ligase

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: EL0013

5000 UNIT MODIFYING ENZYME, LIGASES, T4 DNA LIGASE, 30u/uL, supplied with 5 x 1.5mL 10X T4 DNA

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Product Code. 10548730

Quantity Price
1 £ 185.0 / 5000 units
EU Stock 32
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Description and Specification

Specification

Inhibitors T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration exceeds 200mM.
Concentration 30U/µL
Units HC; 5,000U
Concentration 30U/µL
Molecular Weight 55.3 kDa monomer
Units HC; 5,000U
Source E.coli cells with a cloned gene 30 of bacteriophage T4
Item Description 30u/uL, 5000U

Highlights

  • Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP)
  • Fast – sticky-end ligation is completed in 10 minutes at room temperature
  • Supplied with PEG solution for efficient blunt-end ligation

Applications

  • Cloning of restriction enzyme generated DNA fragments
  • Cloning of PCR products
  • Joining of double-stranded oligonucleotide linkers or adaptors to DNA
  • Site-directed mutagenesis
  • Amplified fragment length polymorphism (AFLP)
  • Ligase-mediated RNA detection (see Reference 3)
  • Nick repair in duplex DNA, RNA or DNA/RNA hybrids
  • Self-circularization of linear DNA.

Notes

  • Binding of T4 DNA Ligase to DNA may result in a band shift in agarose gels. To avoid this, incubate samples with 6X DNA Loading Dye & SDS Solution at 70°C for 5 min or 65°C for 10 minutes and chill on ice prior to electrophoresis.
  • The volume of the ligation reaction mixture should not exceed 10% of the competent cell volume in the transformation process.
  • Prior to electro-transformation, remove T4 DNA Ligase from the ligation mixture by spin column or chloroform extraction. The extracted DNA can be further precipitated with ethanol.