FastDigest™ SacI

The FastDigest SacI restriction enzyme recognizes GAGCT^C sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI)

Overview
Brand: Thermo Scientific

Manufacturer Part Number: FD1133

100 UL RESTRICTION ENZYME, FASTDIGEST SACI, CUTSGAGCT¬C, 1µL cleaves 1µg DNA in 5 minutes at 37°C

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 10324720

Quantity Price
1 £ 24.13 / 100 reactions
EU Stock 50
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Description and Specification

Specification

Components 10X FastDigest Buffer and 10X FastDigest Green Buffer
Product Name FastDigest Sacl
Quantity 100µL
For Use With (Application) Fast clone analysis; Fast preparation of DNA for cloning; Digestion of PCR products; Fast RFLP genotyping; Digestion of difficult-to-cleave DNA
Product Name FastDigest Sacl
Quantity 100µL
Applications Fast clone analysis; Fast preparation of DNA for cloning; Digestion of PCR products; Fast RFLP genotyping; Digestion of difficult-to-cleave DNA
Item Description 100μL

Features

  • 100% activity of all FastDigest enzymes in the universal buffer
  • 100% buffer compatibility with downstream applications
  • Complete digestion in 5-15 minutes
  • Direct loading on gels
  • No star activity
  • 176 FastDigest enzymes available

Applications

  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP analysis

Note

The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity refer to product specifications.