LAMP and Other Isothermal Amplification Technologies
LAMP and Other Isothermal Amplification Technologies
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Thermo Scientific™ EquiPhi29™ DNA Polymerase
Thermo Scientific EquiPhi29 DNA Polymerase is a proprietary phi29 DNA polymerase mutant developed through in vitro protein evolution.
Shipping Condition | Dry Ice |
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Polymerase | EquiPhi29 DNA Polymerase |
Product Type | Stand-alone enzyme |
Reaction Time | 2 hours |
Concentration | 10 U/μL |
Optimal Reaction Temperature | 42°C |
For Use With (Application) | MDA-WGA,RCA |
Invitrogen™ Lyo-ready Bst DNA Polymerase
Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors.
Shipping Condition | Dry Ice |
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Polymerase | Lyo-ready Bst DNA Polymerase |
Product Type | Stand-alone enzyme |
Reaction Time | ≤10 minutes |
Optimal Reaction Temperature | 65°C |
For Use With (Application) | LAMP,RT-LAMP |
Thermo Scientific™ EquiPhi29™ DNA Amplification Kit
The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).
Shipping Condition | Dry Ice |
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Polymerase | EquiPhi29 DNA Polymerase |
Format | Kit |
Form | Liquid |
Product Type | DNA Amplification Kit |
Reaction Time | Up to 2 hours |
Research Category | Nucleic Acid Analysis,In Vitro Transcription,Cell-free Protein Expression,In Situ Sequencing,SNP Detection |
Optimal Reaction Temperature | 42°C |
For Use With (Application) | RCA,MDA-WGA |
Invitrogen™ Colorimetric ReadiLAMP™ Kit, SARS-CoV-2
The Invitrogen Colorimetric ReadiLAMP Kit for SARS-CoV-2 is a loop-mediated isothermal amplification (LAMP)-based solution for rapid SARS-CoV-2 detection in saliva and nasopharyngeal/nasal swab samples.
Includes | LAMP Buffer (5x), Enzyme Mix (10x), SARS-CoV-2 primer mix (5x), Human Control primer mix (5x), SARS-CoV-2 control RNA |
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Detection Limit | <250 copies/25 μL (Direct LAMP) <100 copies/25 μL (Purified RNA-based LAMP) |
Bead Type | Kit does not contain beads. Recommending the use of RNA extraction MVP II kit which contains magnetic beads |
For Use With (Equipment) | Heating block |
Enzyme | SuperScript IV & proprietary Bst-derived DNA polymerase |
Formulation | Liquid. 5 components included in the kit. |
Sample Type | Saliva or Nasopharyngeal/Nasal Swab |
Color | During reaction color changes from purple to blue |
Sample Volume | Range of sample inputs |
Key Functions | Detects SARS-CoV-2 presence |
For Use With (Application) | SARS-CoV-2 surveilance |
Detection Method | Colorimetric readout |
Thermo Scientific™ phi29 DNA Polymerase (10 U/μL)
Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification.
Shipping Condition | Dry Ice |
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Polymerase | phi29 DNA Polymerase |
Product Type | Stand-alone enzyme |
Reaction Time | 4 hours |
Concentration | 10 U/μL |
Optimal Reaction Temperature | 30°C |
For Use With (Application) | MDA-WGA,RCA |
Thermo Scientific™ Reaction Buffer for phi29 DNA Polymerase (10X)
Thermo Scientific 10X Buffer for phi29 DNA Polymerase is the optimal buffer recommended for use with highly processive phi29 DNA polymerase.
Thermo Scientific™ Bsm DNA Polymerase, large fragment (8 U/μL)
Thermo Scientific Bsm DNA Polymerase, Large Fragment, is equivalent to Bst DNA polymerase, which catalyzes 5' → 3' synthesis of DNA and lacks 5' → 3' and 3' → 5' exonuclease activities.
Invitrogen™ SuperScript™ IV RT-LAMP Master Mix
Invitrogen SuperScript IV RT-LAMP Master Mix is a ready-to-use solution optimized for fast, sensitive, and simple RNA target detection in loop-mediated isothermal amplification (LAMP) reactions.
Shipping Condition | Dry Ice |
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Polymerase | Bst DNA Polymerase |
Includes Label or Dye | SYTO 9 Green Fluorescent Nucleic Acid Stain |
Reverse Transcriptase | SuperScript IV |
Product Type | Master Mix |
Reaction Time | 5 to 15 minutes |
Concentration | 2X |
Optimal Reaction Temperature | 65°C |
For Use With (Application) | LAMP,RT-LAMP |