Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

Product Code. 30245142

Brand: Invitrogen™ A72127

Description

Invitrogen Lyo-ready RPA reagents are designed for fast, sensitive, and specific DNA amplification through isothermal recombinase polymerase amplification (RPA) reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.

Invitrogen Lyo-ready recombinase polymerase amplification (RPA) reagents are designed for fast, sensitive, and specific DNA amplification through isothermal RPA reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats. Reverse transcriptase can be added to the RPA reaction for amplification of RNA targets. All reagents are glycerol-free, which enables assay lyophilization.

Features of Lyo-ready RPA products

• Enhanced sensitivity down to 1 copy using optimized (RT-)RPA reaction buffer
• Robust and specific RNA/DNA amplification
• Amplification from inhibitor-containing samples
• Fast target amplification in as little as 20 minutes
• Ability to easily optimize RPA or RT-RPA reactions

Kit components

The Lyo-ready RPA Kit includes all required components for an RPA reaction:

• Lyo-ready T4 UvsX Protein—produced from E. coli cells bearing cloned uvsX gene derived from bacteriophage T4
• Lyo-ready T4 UvsY Protein—a recombination mediator protein that stimulates homologous recombination performed by the UvsX recombinase
• Lyo-ready Bst DNA Polymerase (Cat. No. A56655)—a proprietary mutant of Bst DNA Polymerase, Large Fragment, with fast reaction speed, increased sensitivity, and tolerance to inhibitors
• Lyo-ready T4 Gene 32 Protein—a single-stranded DNA binding protein produced from E. coli cells carrying cloned bacteriophage T4 gene 32
• Optimized reaction buffer, magnesium chloride, and dNTPs

Specifications

• Product Type: Lyo-ready RPA Kit
• Form: Liquid
• For Use With (Application): RPA, RT-RPA
• Polymerase: Lyo-ready Bst DNA Polymerase
• Quantity: 100 Reactions
• Research Category: Nucleic Acid Amplification
• Content And Storage:

  • Lyo-ready T4 UvsX Protein (1.2 mg/mL), 50 μL
  • Lyo-ready T4 UvsY Protein (1.2 mg/mL), 50 μL
  • Lyo-ready Bst DNA Polymerase (6 U/μL), 50 μL
  • Lyo-ready T4 Gene 32 Protein (16 mg/mL), 50 μL
  • 2X RPA Reaction Buffer, 1000 μL
  • 280 mM MgCl₂, 100 μL
  • 10 mM dNTP Mix, 50 μL
  
  Store at -25°C to -15°C.

• Optimal Reaction Temperature: 42°C
• Reverse Transcriptase: SuperScript IV Reverse Transcriptase
• Shipping Condition: Dry Ice
• Detection Method: End point, real-time, lateral flow
• Reaction Time: Up to 20 min.

For Research Use Only. Not for use in diagnostic procedures.