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Reverse Transcription Products

Reverse Transcription Products

A variety of kits, reagents, and mixes suitable for reverse transcription processes. Products include one-step RT-PCR reagent, cDNA synthesis kits and master mixes, and standalone reverse transcriptases.
cDNA Synthesis Kits and Master Mixes (38)
One-Step Reverse Transcription PCR Reagents (13)
Reverse Transcriptases (12)

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1 – 15 of 29 results
1

Invitrogen™ SuperScript™ III Reverse Transcriptase

Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.

Promotions are available
EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript III
Final Product Type First-Strand cDNA
Concentration 200 U/μL
Format Stand-alone Enzyme
Size (Final Product) Up to 12.3 kb
Reaction Format Separate components
Reaction Speed 30 to 50 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
2

Invitrogen™ SuperScript™ II Reverse Transcriptase

Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT.

Promotions are available
Shipping Condition Wet Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript II
Final Product Type First-Strand cDNA
Concentration 200 U/μL
Format Stand-alone Enzyme
Size (Final Product) Up to 12.3 kb
Reaction Format Separate components
Reaction Speed 50 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
3

Invitrogen™ SuperScript™ IV VILO™ Master Mix

SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.

Promotions are available
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript IV
Final Product Type First-Strand cDNA
Concentration 5X
For Use With (Application) Real Time PCR (qPCR)
Detection Method qPCR
Format Master Mix
Size (Final Product) Up to 10 kb
Reaction Format Master Mix
Reaction Speed 10 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
4

Invitrogen™ M-MLV Reverse Transcriptase (200 U/μL)

M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid.

Promotions are available
Shipping Condition Wet Ice
Format Stand-alone Enzyme
Reverse Transcriptase M-MLV
Size (Final Product) Up to 7 kb
Reaction Format Separate components
Final Product Type First-Strand cDNA
Reaction Speed 50 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Concentration 200 U/μL
Optimal Reaction Temperature 37°C
Technique Reverse Transcription
Starting Material RNA
5

Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR.

Promotions are available
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript III
Final Product Type PCR Amplified cDNA
PCR Method 1-step RT-PCR
Detection Method Gel Electrophoresis
Polymerase Platinum Taq DNA Polymerase
Format One-Step RT-PCR System
Size (Final Product) Up to 4.5 kb
Reaction Format Premixed Components
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Hot Start Built-In Hot Start
Optimal Reaction Temperature 50°C
Technique 1-Step RT-PCR
Starting Material RNA
6

Invitrogen™ MultiScribe™ Reverse Transcriptase

MultiScribe Reverse Transcriptase is a recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase optimized for TaqMan-based assays.

Promotions are available
7

Invitrogen™ SuperScript™ IV Reverse Transcriptase

SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions.

Promotions are available
EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript IV
Final Product Type First-Strand cDNA
Concentration 200 U/μL
Format Stand-alone Enzyme
Size (Final Product) Up to 12.3 kb
Reaction Format Separate components
Reaction Speed 10 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
8

Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.

Promotions are available
Shipping Condition Dry Ice
Format Kit
Reverse Transcriptase MultiScribe
Size (Final Product) Up to 7 kb
Reaction Format Premixed Components
Final Product Type First-Strand cDNA
Reaction Speed 30 to 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 37°C
Technique Reverse Transcription
Starting Material RNA
For Use With (Application) Real Time PCR (qPCR),RT-PCR
9

Invitrogen™ Second Strand cDNA Synthesis Kit

Invitrogen™ Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.

Reaction Format Separate Components
Final Product Type Double Stranded cDNA
Product Type cDNA Synthesis Kit
For Use With (Application) Cloning
10

Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix for qRT-PCR

SuperScript III First-Strand Synthesis SuperMix for qRT-PCR provides the high-temperature capability of SuperScript III Reverse Transcriptase in an optimized SuperMix format for the synthesis of first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR).

Promotions are available
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Format Super Mix
Reverse Transcriptase SuperScript III
Reaction Format Master Mix
Final Product Type First-Strand cDNA
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
For Use With (Application) Real Time PCR (qPCR)
Detection Method qPCR
11

Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix

The SuperScript III First-Strand Synthesis SuperMix is an optimized formulation for first-strand cDNA synthesis from purified poly(A)+ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system.

Promotions are available
12

Invitrogen™ SuperScript™ VILO™ Master Mix

SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications.

Promotions are available
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript III
Final Product Type First-Strand cDNA
Concentration 5X
For Use With (Application) Real Time PCR (qPCR)
Detection Method qPCR
Format Master Mix
Size (Final Product) Up to 10 kb
Reaction Format Master Mix
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
13

Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes.

Promotions are available
EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript III
Final Product Type First-Strand cDNA
For Use With (Application) Real Time PCR (qPCR)
Detection Method qPCR
Format Kit
Size (Final Product) Up to 10 kb
Reaction Format Master Mix
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
14

Invitrogen™ SuperScript™ IV First-Strand Synthesis System

The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.

Promotions are available
EDGE
GC-Rich PCR Performance High
Format Kit
Reverse Transcriptase SuperScript IV
Size (Final Product) Up to 12.3 kb
Reaction Format Separate components
Final Product Type First-Strand cDNA
Reaction Speed 10 min.
Reagent Type Reverse Transcription
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
15

Applied Biosystems™ TaqMan™ Reverse Transcription Reagents

TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.

Promotions are available
Shipping Condition Dry Ice
Format Kit
Reverse Transcriptase MultiScribe
Size (Final Product) Up to 5 kb
Reaction Format Separate components
Final Product Type First-Strand cDNA
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Concentration 50 U/μL
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
For Use With (Application) Real Time PCR (qPCR)