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Invitrogen™ SuperScript™ IV VILO™ Master Mix

SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.

£608.00 - £4420.00

Specifications

Detection Method qPCR
Format Master Mix
GC-Rich PCR Performance High
Reaction Speed 10 min.
Technique Reverse Transcription
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Products 3
Product Code Brand Includes No. of Reactions Price Quantity & Availability  
Product Code Brand Includes No. of Reactions Price Quantity & Availability  
15574395
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Invitrogen™
11756050
Kit only 50 Reactions
£608.00
50 reactions
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15584395
View Documents Promotion Details
Invitrogen™
11756500
Kit only 500 Reactions
£4420.00
500 reactions
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15523145
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Invitrogen™
11766050
Kit with ezDnase 50 Reactions
£660.00
50 reactions
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Description

Description

SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications. The inclusion of ezDNase enzyme further accelerates the RT-qPCR workflow through an extremely simplified genomic DNA removal step.

SuperScript IV VILO Master Mix features include:
• Fast RT reactions in just 10 minutes
• Convenient one-tube cDNA reaction master mix for two-step RT-qPCR
• High yields reduce Ct values by more than two cycles ahead of all other reverse transcription reagents
• Efficient reaction even with low template amounts and suboptimal purity samples
• gDNA removal in as few as two minutes (SSIV VILO Master Mix with ezDNase only)

SSIV VILO Master Mix elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV Reverse Transcriptase and further optimized buffer. These components enable efficient cDNA synthesis at higher temperatures and in less time. SSIV VILO master mix provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates. It is your new tool for more efficient and reproducible RT-qPCR.

SuperScript IV VILO Master Mix with ezDNase is available for better removal of genomic DNA and to further accelerate the RT-qPCR workflow. The extremely simplified genomic DNA removal step dramatically reduces the time of the entire reverse transcription protocol and reduces possible variation in gene expression due to RNA loss or damage during conventional DNase treatment.

SSIV VILO master mix results in more cDNA in less time with less pipetting, less variation, less reaction inhibition, less gDNA interference, and with less sample than other cDNA synthesis kits or master mixes for RT-qPCR. Additional ezDNase may also be purchased separately.

Source
Purified from E. coli expressing the pol gene of M-MLV, modified to increase thermostability and half-life, processivity, inhibitor resistance, and to reduce RNase H activity

Performance and quality testing
Assayed for endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease, as well as yield and length of cDNA product

Specifications

Specifications

qPCR
High
Reverse Transcription
SuperScript IV
Dry Ice
5X
Master Mix
Up to 10 kb
Master Mix
10 min.
50°C
Reduced
Real Time PCR (qPCR)
First-Strand cDNA
Reverse Transcription
RNA
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.