Affinity Gels
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Cytiva Protein G Sepharose™ 4 Fast Flow Media
Rapid processing of large volumes, recoveries of 70 to 90%. Cytiva Protein G Sepharose™ 4 Fast Flow Media is recombinant protein G coupled to Sepharose 4 Fast Flow.
| Particle Size | 90 μm |
|---|---|
| Content And Storage | 2°C to 8°C |
| Matrix | 4% Cross Linked Agarose |
| Ligand | Recombinant Protein G lacking albumin binding region |
| Ligand Concentration | 2mg Protein G/mL Medium |
| pH | 3 to 9 |
| For Use With (Application) | Used in a range of diagnostic and research applications |
Cytiva EAH Sepharose™ 4B Medium
Used for coupling compounds containing carboxyl groups to Sepharose 4B. EAH Sepharose™ 4B Medium provides extremely stable coupling via an 11-atom spacer arm.
Cytiva CNBr-Activated Sepharose™ Medium
Use for coupling antibodies or other large proteins containing -NH2 groups to the Sepharose media. CNBr-Activated Sepharose™ Medium is a pre-activated media perfect for custom affinity applications.
Cytiva Calmodulin Sepharose™ 4B Medium
Use for purification of proteins with affinity for calmodulin, a highly conserved regulatory protein involved in many cellular processes in eukaryotic cells. Calmodulin Sepharose™ 4B Medium provides single-step purification.
Cytiva Lentil Lectin Sepharose™ 4B Medium
Routinely used in the preparation and purification of a number of glycoproteins and carbohydrate-containing molecules
Cytiva Protein G Mag Sepharose™ Xtra
High binding capacity, 27mg human IgG/mL medium, for efficient capture of antibody with high yield and purity
| Content And Storage | 4°C to 8°C |
|---|---|
| Matrix | Paramagnetic, spherical, highly cross-linked agarose particles |
| Bead Type | Protein G Mag Sepharose Xtra magnetic bead |
| Ligand | Protein G |
| Binding Capacity | 27mg human IgG/mL gel |
| For Use With (Application) | For rapid, small-scale purification and screening of monoclonal and polyclonal antibodies from serum and cell supernatants |
Cytiva Epoxy-activated Sepharose™ 6B Medium
A pre-activated medium for immobilization of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose 6B via a 12-atom hydrophilic spacer arm
Cytiva Con A Sepharose™ 4B Medium
An affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids
| Particle Size | 90 μm |
|---|---|
| Content And Storage | 2°C to 8°C |
| Matrix | 4% Agarose |
| Ligand | Concanavalin A |
| Ligand Concentration | 13mg Con A/mL Drained Medium |
| Binding Capacity | 30mg/mL |
| pH | 4 to 9 |
| For Use With (Application) | For separation and purification of Glycoproteins, Polysaccharides and Glycolipids, isolates cell surface Glycoproteins from detergent-solubilized membranes |
Cytiva Protein A and Protein G Mag Sepharose™
Simplify enrichment of target proteins by immunoprecipitation or pull-down applications
| Particle Size | 37 to 100 μm |
|---|---|
| Content And Storage | 4°C to 8°C |
| Matrix | Paramagnetic spherical, highly cross linked agarose particles |
Cytiva NHS Mag Sepharose™
NHS Mag Sepharose magnetic beads for convenient enrichment of selected proteins using pull-down sample preparation techniques
| Particle Size | 37 to 100 μm |
|---|---|
| Content And Storage | 4°C to 8°C |
| Matrix | Paramagnetic spherical, highly cross linked agarose particles |
| Bead Type | NHS Mag Sepharose |
| Ligand | N-hydroxysuccinimide |
| Ligand Concentration | 8 to 14μM/mL |
Cytiva Activated Thiol Sepharose™ 4B Medium
For reversible coupling of proteins to Sepharose 4B via a glutathione spacer arm
Cytiva 2'5' ADP Sepharose™ 4B medium
2'5' ADP is an NADP structural analog immobilized on Sepharose 4B
Cytiva ECH Sepharose™ 4B Medium
A pre-activated media for coupling of compounds with amino groups to Sepharose 4B via a 10-atom hydrophilic spacer arm by the epoxy coupling method
Cytiva Protein A Sepharose™ CL-4B Medium
For immunoglobin purification and fractionation. Cytiva Protein A Sepharose™ CL-4B Medium is also well suited for immunoprecipitation.
| Particle Size | 90 μm |
|---|---|
| Content And Storage | 2°C to 8°C |
| Matrix | 4% Cross Linked Agarose |
| Ligand | Native Protein A |
| Ligand Concentration | 3mg Protein A/mL Medium |
| Binding Capacity | 20mg Human IgG/mL |
| pH | 2 to 10 |
| For Use With (Application) | For immunoglobin purification and fractionation and is suitable for immunoprecipitation procedures |
Cytiva TiO2 Mag Sepharose™
Designed for enrichment of phosphopeptides from tryptic digested protein samples. Cytiva TiO2 Mag Sepharose magnetic beads are based on titanium dioxide chromatography and are designed to simplify capture and enrichment of phosphopeptides.
| Particle Size | 37 to 100 μm |
|---|---|
| Content And Storage | 4°C to 30°C (Use at room temperature) |
| Matrix | Paramagnetic spherical, highly cross linked agarose particles |
| Bead Type | TiO2 Mag Sepharose |
| Ligand | TiO2 |
| Storage Buffer | 20% ethanol storage solution |
| For Use With (Application) | Used for selective capture and enrichment of Phosphopeptides, for biomarker research |