Cell Based Assays
Invitrogen™ Transferrin From Human Serum, Alexa Fluor™ 647 Conjugate
Labeled LDL complexes useful in studying the delivery of up to two Fe3+ atoms via receptor-mediated endocytosis
Invitrogen™ NucBlue™ Fixed Cell ReadyProbes™ Reagent (DAPI)
High-purity form of DAPI stain in room temperature-stable solution that is provided in dropper bottle
Molecular Probes™ Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit
Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit
Thermo Scientific™ Mammalian beta-Galactosidase Assay Kit
Lyse cultured mammalian cells and measure beta-galactosidase activity using compatible M-PER Cell Lysis Reagent and colorimetric b-Gal Assay Reagent.
Invitrogen™ Dextran, Alexa Fluor™ 680; 3,000 MW, Anionic
Rigorous methods for removing as much unconjugated dye as practical, followed by assay of dextran conjugates by thin-layer chromatography to help ensure absence of low molecular weight contaminants
Invitrogen™ DiD' solid; DiIC18(5) solid (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate Salt)
When applied to cells, dye diffuses laterally within the plasma membrane
Invitrogen™ Transferrin From Human Serum, Alexa Fluor™ 488 Conjugate
Transferrin is a monomeric serum glycoprotein that binds to a specific receptor on the surface of vertebrate cells and delivers up to two Fe3+ atoms via receptor-mediated endocytosis—our labeled LDL complexes are useful tools for studying this phenomenon.
Invitrogen™ 5-CFDA, AM (5-Carboxyfluorescein Diacetate, Acetoxymethyl Ester)
A cell-permeant esterase substrate that can serve as a viability probe
Invitrogen™ Dextran, Alexa Fluor™ 555; 10,000 MW, Anionic, Fixable
Rigorous methods for removing as much unconjugated dye as practical, followed by assay of dextran conjugates by thin-layer chromatography to help ensure absence of low molecular weight contaminants
Invitrogen™ Dextran, Texas Red™, 70,000 MW, Neutral
Rigorous methods for removing as much unconjugated dye as practical, followed by an assay of dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants
Invitrogen™ Dil Stain (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate ('DiI'; DiIC18(3)))
DiI is a lipophilic membrane stain that diffuses laterally to stain the entire cell. It is weakly fluorescent until incorporated into membranes.
Invitrogen™ Dextran, Tetramethylrhodamine, 70,000 MW, Lysine Fixable
Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the hydrodynamic properties of the cytoplasmic matrix.
Invitrogen™ DiR'; DiIC18(7) (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindotricarbocyanine Iodide)
The near IR fluorescent, lipophilic carbocyanine DiOC18(7) ('DiR') is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes.
Invitrogen™ Dextran, Cascade Blue™, 10,000 MW, Anionic, Lysine Fixable
Rigorous methods for removing as much unconjugated dye as practical, followed by an assay of dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants
Invitrogen™ Dextran, Fluorescein, 70,000 MW, Anionic, Lysine Fixable
Rigorous methods for removing as much unconjugated dye as practical, followed by an assay of dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants