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Direct PCR Reagents and Kits

PCR products that allow for the amplification of DNA directly from unpurified source material. Includes various kits, master mixes, enzymes, and reagents.
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1 – 15 of 23 results
1

Thermo Scientific™ ABsolute™ qPCR Master Mixes, SYBR Green-Based Detection

Thermo Scientific™ ABsolute qPCR Master Mixes offer sensitivity and reproducibility for real-time PCR applications.

Activation Time heated to 95°C for 15 minutes
2

ABsolute™ qPCR Master Mixes, SYBR Green-Based Detection

Thermo Scientific™ ABsolute qPCR Master Mixes offer sensitivity and reproducibility for real-time PCR applications.

3

Verso™ 1-Step SYBR Green-Based Detection RT-qPCR Kits

Thermo Scientific™ Verso™ 1-Step RT-qPCR Kits have been specially developed to quantify RNA in a convenient one-step assay.

4
Promotions Available

Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
GREENER_CHOICE

The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

EDGE
Shipping Condition Frozen, Dry Ice
Content And Storage Express Lysis Solution (room temperature or 4°C), Express ezDNase (-20°C), SuperScript IV VILO Master Mix (-20°C), SuperScript IV VILO No RT Control (-20°C), TaqMan Fast Advanced Master Mix (4°C)
Isolation Technology Direct cell lysis
Kit Type Direct Cell Lysis RT-qPCR
Green Features REACH compliant, less waste
For Use With (Application) Gene Expression
High-throughput Compatibility Yes
PCR Method 2-step RT-qPCR
Detection Method TaqMan
Target cDNA
Format Kit
Sample Type Mammalian Cells
Purification Target RNA
Starting Material 10-100,000 Cells
5
Promotions Available

Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit
GREENER_CHOICE

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

EDGE
GC-Rich PCR Performance High
Format Tube
Product Type 1-Step qPCR kit
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Mammalian Cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Green Features Fewer resources used and less waste, Less hazardous
Starting Material 10-100,000 Cells
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Product Line Cells-to-CT, TaqMan
Detection Method TaqMan
6

Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit
GREENER_CHOICE

The SYBR Green Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2X
Green Features Fewer resources used and less waste, Less hazardous
Concentration 2X
For Use With (Application) Gene Expression
High-throughput Compatibility High-throughput Compatible
PCR Method 2-step RT-qPCR
Detection Method SYBR
Format Tube
Product Type Fast Advanced Cells-to-Ct Kit
Sample Type Mammalian Cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Purification Target Lysate
Starting Material 10-100,000 Cells
Product Line Cells-to-CT
7

Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit
GREENER_CHOICE

The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2X
Green Features Fewer resources used, less waste, less hazardous
Concentration 2X
For Use With (Application) Gene Expression
High-throughput Compatibility High-throughput Compatible
PCR Method 2-step RT-qPCR
Detection Method TaqMan
Format Tube
Product Type Fast Advanced Cells-to-Ct Kit
Sample Type Mammalian cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Purification Target Lysate
Starting Material 10-100,000 Cells
Product Line Cells-to-CT
8

Invitrogen™ TaqMan™ Cells-to-CT™ Control Kit
GREENER_CHOICE

The Invitrogen™ TaqMan™ Cells-to-CT Control Kit is designed for use with the TaqMan™ Gene Expression Cells-to-CT Kit.

9

Invitrogen™ Single Cell Lysis Kit
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Single-cell analysis has the potential to transform life science research. However, detecting and quantitating the minute amounts of RNA contained in single cells remains a significant challenge.

10

Invitrogen™ Cells-to-cDNA™ II Kit

The Ambion™ Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr., no RNA isolation is required.

Shipping Condition Dry Ice
Content And Storage 1X PBS, Cell Lysis II Buffer, μL DNase 1, 10X RT Buffer, M-mLV Reverse Transcriptase, RNase Inhibitor, dNTP Mix, Random Decamers, Oligo(dT)18 Primers, Armored RNA™ Control, Armored RNA™ Primer Pair, and Endogenous Primer Pair should be stored at
-20°C. Nuclease-free water may be stored at any temperature.
Reverse Transcriptase M-MLV
Size (Final Product) 7 kb or less
Final Product Type cDNA
Sample Type Mammalian Cells
Purification Time 2 hr.
Optimal Reaction Temperature 42°C
For Use With (Application) Real Time PCR (qPCR),RT-PCR
Product Line Ambion, Cells-to-cDNA
11
Promotions Available

Thermo Scientific™ GeneJET PCR Purification Kit

Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

Content And Storage For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
Format Spin Column
Run Time 5 min.
High-throughput Compatibility Not High-throughput Compatible (Manual)
12

Invitrogen™ TaqMan™ Gene Expression Cells-to-CT™ Kit
GREENER_CHOICE

The TaqMan™ Gene Expression Cells-to-CT™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Purity or Quality Grade UP (Ultra Pure)
Reverse Transcriptase M-MLV
Green Features Fewer resources used and less waste, Less hazardous
PCR Method 2-step RT-qPCR
Detection Method Primer-probe
Polymerase DNA Polymerase
Format Tube
Product Type Gene Expression Cells-to-C Kit
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Cell
Reaction Speed Standard
Product Line Ambion, Cells-to-CT, TaqMan
13

Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit
GREENER_CHOICE

The Ambion Single Cell-to-CT™ Kit contains a complete validated workflow for gene expression analysis for samples containing 1–10 cells.

Shipping Condition Dry Ice
Sample Type Mammalian Cells
Reaction Speed 7 min.
Purification Time 7 min.
Green Features Fewer resources used and less waste, less hazardous
Starting Material 1-10 Cells
For Use With (Application) RT-PCR
PCR Method 2-step RT-qPCR
Product Line Ambion, Cells-to-CT
Detection Method TaqMan
14

Applied Biosystems™ MagMAX™ Viral/Pathogen Binding Beads

These binding beads are included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit.

15

Invitrogen™ TaqMan™ PreAmp Cells-to-CT™ Kit
GREENER_CHOICE

The TaqMan PreAmp Cells-to-CT Kit makes it possible to perform gene expression analysis directly from limited or small numbers of cultured cells without RNA purification.