Verso™ 1-Step SYBR Green-Based Detection RT-qPCR Kits

Product Code. 11872143

Brand: Thermo Scientific ABgene AB4106C

Description

Thermo Scientific™ Verso™ 1-Step RT-qPCR Kits have been specially developed to quantify RNA in a convenient one-step assay.

Quantify RNA in a convenient one-step assay with Thermo Scientific™ Verso™ 1-Step SYBR Green-Based Detection RT-qPCR Kits.

Verso 1-Step Kits are designed for minimal hands-on time and faster results. The RT and QPCR reaction components are together in a single tube, reducing protocol times and minimizing the number of pipetting steps. The buffer system of the Verso 1-Step QRT-PCR Kits has been specifically optimized to work across a wide range of RNA templates for both transcription and amplification. Special enhancers and additives have been used to generate a reaction mixture which allows both reverse transcriptase and DNA polymerase to work optimally.

• Optimized buffer—Verso RT-qPCR Kits are based on a balanced buffer to maximize performance of both the reverse transcriptase and DNA polymerase enzymes

• Easy-to-see mix—The Verso RT-qPCR Kit contains an inert blue dye for easy sample visualization during reaction set-up

• Superior dynamic range—Kit enables the detection of 10pg to 1Glc mug of total RNA or 1pg to 10ng mRNA, allowing for accurate detection and quantification of target RNA over a broad template range and ensuring that increases in target RNA concentration give rise to corresponding increases in qPCR signal

• Maximum specificity—The chemically modified Thermo-Start™ Taq DNA Polymerase remains completely inactive during the reverse transcription stage; the enzyme is then fully activated at the start of the qPCR reaction by a 95°C heating step

• Broad template range—Verso 1-Step RT-qPCR Kit can detect 10pg to 1μg of total RNA or 1pg to 10ng of mRNA

• Easily adapted for automation—The simple 1-step protocol is compatible with robotic systems and reduces hands-on time

• Unique RT Enhancer—Verso RT-qPCR Kits contain RT Enhancer, a unique enzyme that degrades any contaminating genomic DNA during the RT step and eliminates the need for a DNase I treatment of the extracted RNA, saving time and effort

• Instrument specific—Individually optimized qPCR mixes designed for all major qPCR / Real-Time PCR instruments

• Reproducible results—All mixes undergo extensive quality control testing to ensure batch-to-batch consistency

Includes:

Verso Enzyme Mix (RNase inhibitor, RT Enhancer, Thermo-Start™ Taq DNA Polymerase‡, High performance dNTPS, SYBR Green I, ROX™ passive reference dye (only in ROX mixes), Fluorescein (only in Fluorescein mix)); Separate MgCl₂ vial for further MgCl₂ optimization; Separate ROX vial (only in kits indicated)

Compatible with:

These blue mixes are ideal for use with white reaction plates and tubes. An increase in detection efficiency is seen when using ABgene white PCR plastics for qPCR. While white plastics give superior qPCR results, they can make traditional colorless reagents difficult to see. With Verso, a simple glance ensures that the correct amount of master mix has been added.

Check qPCR cycler compatibility with our qPCR reagent selection guide.

Recommended for:

Ideal for a range of applications including gene expression analysis, siRNA knockdown validation, miRNA detection, microarray validation, pathogen detection and disease research

Specifications

• Content And Storage: Verso Enzyme Mix (RNase inhibitor, RT Enhancer, Thermo-Start™ Taq DNA Polymerase‡, High performance dNTPS, SYBR Green I, ROX™ passive reference dye (only in ROX mixes), Fluorescein (only in Fluorescein mix)); Separate MgCl2 vial for further MgCl2 optimization; Separate ROX vial (only in kits indicated). Store at -20°C. Avoid freeze/thaw cycles. Protect from light
• Description: With Low ROX
• Product Type: 1-Step QRT-PCR Kit w/SYBR Green and Low ROX
• Quantity: 400 x 25µL Rxns
• Sufficient For: 400 samples
• For Use With (Application): Gene Expression, siRNA Validation, miRNA analysis, Microarray Analysis, Pathogen Detection, Genetic Disease Research

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.