Thermo Scientific™ GeneJET PCR Purification Kit

Product Code. 10400450

Brand: Thermo Scientific™ K0701

Description

Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions. It effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures.

The kit can be used for purification of DNA fragments from 25 bp to 20 kb with recovery rates up to 100%. Each GeneJET purification column has a total binding capacity of up to 25 μg of DNA, and the entire procedure takes 5 minutes. The purified DNA can be used in common downstream applications, such as sequencing, restriction digestion, labeling, ligation, cloning, in vitro transcription, blotting or in situ hybridization.

Highlights

• Highly efficient: 90 to 100% recoveries in the range of 100 bp to 10 kb
• Fast: procedure takes only 5 minutes
• Convenient: spin columns are capped and assembled with collection tubes

Applications

Fast and efficient purification of DNA fragments ideal for use in all conventional molecular biology procedures including:

• FastDigest or conventional restriction digestion
• Automated fluorescent or radioactive sequencing
• PCR
• In vitro transcription
• In situ hybridization
• Labeling
• Cloning

Specifications

• Content And Storage: For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
• Format: Spin Column
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Product Line: GeneJET
• Product Type: PCR Purification Kit
• Quantity: 50 Preps
• Run Time: 5 min.
• Target: DNA from Enzymatic Reactions (e.g. PCR)
• For Use With (Application): Real Time PCR (qPCR), Cloning, DNA Sequencing
• Isolation Technology: Spin Column
• Sample Type: dsDNA

Safety and Handling

Product Identifier

• genejet PCR purif

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Skin corrosion/irritation Category 1 C

Hazard Statement

• H302-Harmful if swallowed.
• H314-Causes severe skin burns and eye damage.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P330-Rinse mouth.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : thiocyanic acid

For Research Use Only. Not for use in diagnostic procedures.