MACHEREY-NAGEL – Products for thin layer chromatography (TLC/HPTLC)

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MACHEREY-NAGEL and Chromatography

We provide optimal and reliable solutions for your laboratory work in sample preparation, method development and routine analysis.

About Us

MACHEREY-NAGEL Chromatography – complete solutions for your analysis

From a pioneer in chromatography to a full-range supplier of laboratory consumables, we supply laboratories around the world with HPLC, GC and SPE columns, TLC/HPTLC plates, autosampler vials, closures and more.

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MACHEREY-NAGEL offers a versatile range of ready-to-use TLC and HPTLC layers, which are the result of 50 years of continuous research and development in thin layer chromatography.


The comprehensive range of TLC products from MACHEREY-NAGEL includes:

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TLC/HPTLC glass plates

ADAMANT, SIL G or SIL HD are silica layers on glass backing. It’s hard to imagine a laboratory without our reliable TLC and HPTLC glass plates.

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ALUGRAM and POLYGRAM TLC/HPTLC sheets are easy to handle, lightweight and flexible.

High performance silica on ALUGRAM Xtra sheets provides outstanding wettability for precise colourisation results. Moreover these TLC sheets are easy to cut with scissors.

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Accessories

In addition to high quality TLC plates and sheets, we offer staining reagents and developing chambers, as well as introductory sets for educational purposes.

TLC Backings & Supports
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TLC/HPTLC plates and sheets consist of an adsorbent stationary phase on a backing support.

MACHEREY-NAGEL offers three different backings:
 

  • Glass plates
  • Aluminium sheets
  • Polyester sheets

Properties of TLC backings

Glass Plates

  • Thickness (approx.): 1.3mm
  • Weight and storage requirements: high
  • Torsional strength: ideal
  • Temperature stability: high
  • Susceptible to breakage: yes
  • Can be cut with scissors: no
  • Chemical resistance against solvents: high
  • Chemical resistance against mineral acids and concentrated ammonia: high
  • Suitability for aqueous detection reagents: depends on phases (layer)

Glass plates are robust, heat proof and chemically resistant to all common mobile phases and visualisation reagents. They are the traditional option in TLC/HPTLC and are widely used in the pharmaceutical sector and other laboratory environments.


Aluminium Sheets - ALUGRAM Xtra

  • Thickness (approx.): 0.15mm
  • Weight and storage requirements: low
  • Torsional strength: relatively high
  • Temperature stability: high
  • Susceptible to breakage: no
  • Can be cut with scissors: yes
  • Chemical resistance against solvents: high
  • Chemical resistance against mineral acids and concentrated ammonia: low
  • Suitability for aqueous detection reagents: ALUGRAM: low to moderate, ALUGRAM Xtra: moderate to high

Aluminium sheets are easy to handle, lightweight and flexible. High performance silica on ALUGRAM Xtra sheets provides outstanding wettability for precise colourisation results, even with 100% aqueous detection reagents. Moreover, ALUGRAM Xtra sheets are easy to cut with scissors. No flaking of silica occurs!


Polyester Sheets – POLYGRAM

  • Thickness (approx.): 0.2mm
  • Weight and storage requirements: low
  • Torsional strength: low
  • Temperature stability: maximum 185°C
  • Susceptible to breakage: no
  • Can be cut with scissors: yes
  • Chemical resistance against solvents: high
  • Chemical resistance against mineral acids and concentrated ammonia: high
  • Suitability for aqueous detection reagents: good

Polyester sheets are easy to handle, lightweight and flexible, and can also be cut with scissors. Developed POLYGRAM sheets can also be stored for documentation in laboratory notebooks.

TLC and HPTLC
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Features of modern TLC/HPTLC

The success of thin layer chromatography as a highly efficient microanalytical separation method is based on a large number of advantageous properties:

  • High sample throughput in a short time
  • Suitable for screening tests
  • Pilot procedure for HPLC and Flash chromatography
  • After separation the analytical information can be stored for a longer period of time (the TLC ready-to-use layer acts as storage medium for data)
  • Separated substances can be subjected to subsequent analytical procedures (e.g., IR, MS) at a later date
  • Rapid and cost-efficient optimisation of the separation due to easy change of mobile and stationary phase
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'TLC' and 'HPTLC' are both widely used terms in laboratory environments, but what is the difference? At MACHEREY-NAGEL, we define 'HPTLC' where at least one of the following criteria is being fulfilled:

  • Layers with particle size 2–10 μm
  • Layers prefixed with 'Nano'
  • Modified layers

Narrow fractionation of HPTLC silica particles allows:

  • Sharper separations
  • Shorter developing times
  • Shorter migration distances
  • Smaller samples needed
  • Increased detection sensitivity (compared to TLC)
Products

Macherey-Nagel™ Standard SIL G Silica Layers on Alugram™ Aluminum Sheets UV254

Separate non-volatile mixtures with unmodified standard silica layers for TLC (Thin-Layer Chromatography).  Standard SIL G Silica Layers on Alugram™ Aluminum Sheets UV254 come in a variety of sizes. X20 ALUGRAM-Sheets SIL G/UV 254 thickness: 0.2 µm, size: 10 x 20 cm

Macherey-Nagel™ Quantofix™ Nitrate and Nitrite Test Strip Kits

Allows for the quick and easy determination of nitrate and nitrite in solutions. Macherey Nagel™ Quantofix™ Nitrate and Nitrite Test Strip Kits are semi-quantitative, come pre-calibrated and contain all necessary equipment and reagents.
X100 100 QUANTOFIX TEST STRIPS,FOR THE SEMI QUANTI

Macherey-Nagel™ Tritest™ pH Paper Roll

Featuring three indicator zones for the highest accuracy reading. Macherey Nagel™ Tritest™ pH Paper Rolls give the user the most precise readings possible in pH measurement.
5M PH PAPER 1-11 TRITEST

Macherey-Nagel™ Quantofix™ Peroxide 100 Test Strip Kits

Allows for the quick and easy determination of peroxide levels in solutions. Macherey Nagel™ Quantofix™ Peroxide 100 Test Strip Kits are semi-quantitative, come pre-calibrated and contain all necessary equipment and reagents.
X100 Test sticks QUANTOFIX Peroxide 100 semi,

Macherey-Nagel™ Quantofix™ Ammonium Test Strip Kits

Allows for the quick and easy determination of ammonium in solutions. Macherey Nagel™ Quantofix™ Ammonium Test Strip Kits are semi-quantitative, come pre-calibrated and contain all necessary equipment and reagents. QUANTOFIX Ammonium box of 100test sticks 6 x 95 mm incl. reagents __UN 3316

Macherey-Nagel™ Standard SIL G Silica Layers on Polygram™ polyester sheets

Separate non-volatile mixtures with unmodified standard silica layers for TLC (Thin-Layer Chromatography). Standard SIL G silica layers on Polygram™ polyester sheets come in a variety of sizes. Select fluorescent indicator and choose from a variety of pack sizes. TLC PLATE POLYGRAM(R) SIL G-25UV254 analytical silica 60 on polyester sheet one

Macherey-Nagel™ NucleoSpin™ rDNase Set

Suitable for time-saving on-column DNA removal, as well as for efficient DNA removal in the eluate. Macherey-Nagel™ NucleoSpin™ rDNase Set is highly specific recombinant DNase and reaction buffer for efficient removal of contaminating DNA. Sufficient for 50 mini preparations of total RNA. 1 SET RDNASE SET, INCLUDING RDNASE AND RDNASEreaction buffer for50 minipreps of total RNA

Macherey-Nagel™ Quantofix™ Peroxide 25 Semi-quantitative Test Strips

Ideal to test low levels of peroxide in solutions.  Macherey Nagel™ Quantofix™ Peroxide 25 Semi-quantitative Test Strips use an easy dip-and-read procedure and provide reliable results within 15 seconds. X100 Test sticks QUANTOFIX Peroxide 25 semi,

Macherey-Nagel™ Quantofix™ Formaldehyde Test Strip Kits

Allows for the quick and easy determination of formaldehyde in solutions. Macherey Nagel™ Quantofix™ Formaldehyde Test Strip Kits are semi-quantitative, come pre-calibrated and contain all necessary equipment and reagents. X100 Test sticks QUANTOFIX Formaldehyde semi,

Macherey-Nagel™ NucleoSpin™ RNA Plant Kit

Isolates total RNA from all kinds of plant material and filamentous fungi without degradation of the RNA. Macherey-Nagel™ NucleoSpin™ RNA Plant Kit is a complete mini spin kit that includes RNA plant columns with collection tubes, filters, all needed buffers and RNase-free rDNase. NucleoSpin(R) RNA Plant, sample, 10 preps storeat ambient in dark

Macherey-Nagel™ NucleoSpin™ Plasmid Columns

Prepare small scale high-purity plasmid DNA rapidly. NucleoSpin™ Plasmid Columns come with or without a cap lid. 50 RXN NUCLEOSPIN(R) PLASMID (NOLID), 2ML COLLECTIon tubes, buffers,RNase A 50 preps Store ambiently

Macherey-Nagel™ Quantofix™ Zinc Test Strip Kits

Allows for the quick and easy determination of zinc in solutions. Macherey Nagel™ Quantofix™ Zinc Test Strip Kits come pre-calibrated and contain all necessary equipment and reagents. X100 Test sticks QUANTOFIX Zinc semi, quantitative

Macherey-Nagel™ Glass Laboratory Sprayer

Used as an accessory in thin layer chromatography applications. Macherey-Nagel™ Glass Laboratory Sprayer includes a 100mL, clear glass conical flask with a sprayer-stopper assembly and rubber bulb/tubing. Glass from laboratory sprayer with rubber bulb

Macherey-Nagel™ Weighing Papers

Guarantees that weighed goods can be transferred without loss. Macherey Nagel™ Weighing Papers are transparent and smooth on both sides. X100 Weighing Paper MN 226, 9x11.5cm (100 sheets)

Macherey-Nagel™ Binding Buffer NTI

Used with the NucleoSpin™ Gel and PCR Clean-up Kit. Macherey-Nagel™ Binding Buffer NTI is a standard binding buffer with a pH indicator to ensure optimal binding conditions during DNA gel extraction and PCR clean up. Binding buffer is loaded in a single step. 120ML Binding Buffer NTI

Macherey-Nagel™ NucleoSpin™ Resuspension Buffer A1

For use in NucleoSpin™ Plasmid DNA purification kits for rapid small-scale preparation of high-copy plasmids from E. coli. Macherey-Nagel™ NucleoSpin™ Resuspension Buffer A1 without RNase A is a replacement component used in the NucleoSpin™ method's cell lysis process to resuspend the cell pellet. 1 LT RESUSPENSION BUFFER A1 WITHOUT RNASE A MACHERey-Nagel 1L

Macherey-Nagel™ NucleoBond™ Xtra Combi Rack

Holds up to six (6) NucleoBond™ Xtra Midi and four (4) NucleoBond™ Xtra Maxi Columns. Macherey-Nagel™ NucleoBond™ Xtra Combi Rack is compatible with Xtra Midi, Xtra Maxi, Xtra BAC, AX 20, AX 100, AX 500, AX 2000, AX 10000 and BAC 100 columns. COMBI RACK NUCLEOBOND XTRA

Macherey-Nagel™ Standard SIL G Silica Layers on Glass Plates

Separate non-volatile mixtures with unmodified standard silica layers for TLC (Thin-Layer Chromatography). Standard SIL G silica layers on glass plates come in a variety of plate sizes and layer thicknesses. Select a fluorescent indicator and choose from a variety of pack sizes. X25 TLC precoated plates SIL G-25 UV 254+366 size: 20x20 cm

Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Columns

Clean up PCR Columns for use with NucleoSpin kits. X250 NucleoSpin Extract II column

Macherey-Nagel™ Proteinase K

Delivered with a stabilizing solution for enhanced stability. Macherey-Nagel™ Proteinase K is lyophilized proteinase K supplied in pre-measured vials for easy preparation of 20mg/mL stock solution. It can be used with NucleoSpin™ and NucleoBond™ kits. Proteinase K (30 mg)

Macherey-Nagel™ NucleoBond™ Xtra Maxi Plus EF

Purify endotoxin-free plasmid DNA with high yield. The NucleoBond™ Xtra Maxi Plus EF Kit represents the new generation of anion exchanger allowing high yield, ultra-fast. The Xtra kit contains NucleoBond™ Finalizers to speed up DNA precipitation. NucleoBond Xtra Maxi Plus EF(10) 10 preps for the isolation of endotoxin-free

Macherey-Nagel™ NucleoSpin™ Tissue Column

Purify total DNA. Macherey Nagel™ NucleoSpin™ Column is an all-round column kit with the highest sensitivity for purification of total DNA from clinical or forensic samples, tissues, cells, yeast, bacteria, or viruses NucleoSpin Tissue (10)

Macherey-Nagel™ Chromafix™ PS-H+ SPE Cartridge

For removal or concentration of cations from water, decreasing the pH value of basic samples for standard protocol. Macherey-Nagel™ Chromafix™ PS-H+ SPE Cartridge is a polymer-based SPE phase for RP and ion chromatography. X50 Solid Phase Extraction Cartridges CHROMAFIX PS-H+ (L) average content of sorbent: 900 mg

Macherey-Nagel™ Quantofix™ Copper Test Strip Kits

Allows for the quick and easy determination of copper in solutions. Macherey Nagel™ Quantofix™ Copper Test Strip Kits are semi-quantitative, come pre-calibrated and contain all necessary equipment and reagents.
X100 Test sticks QUANTOFIX Copper semi, quantitati

Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit

Clean up PCR reactions and gel extractions with a two-in-one approach. Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit is optimized for recovery and elution volume when purifying DNA fragments via both applications. NucleoSpin Gel and PCR Clean-up (250)

Macherey-Nagel™ NucleoSpin™ RNA, Mini Kit for RNA purification

Mini spin kit for isolation of RNA of highest integrity. 10 RXN NUCLEOSPIN(R) RNA II, CONTAINS NUCLEOSPINRNA II columns withcollection tubes (2mL/1.5mL),

Macherey-Nagel™ NucleoBond™ Xtra Midi

Purify transfection-grade plasmid DNA with high yield. The NucleoBond™ Xtra Midi Kit represents the new generation of anion exchanger allowing high yield, ultra-fast. NucleoBond Xtra Midi (100)100 preps for the isolation of plasmid DNA,

Macherey-Nagel™ Quantofix™ Nickel Test Strip Kits

Allows for the quick and easy determination of nickel in solutions. Macherey Nagel™ Quantofix™ Nickel Test Strip Kits are semi-quantitative, come pre-calibrated and contain all necessary equipment and reagents. X100 100 QUANTOFIX TEST STRIPS,FOR THE SEMI QUANTI

Macherey-Nagel™ Wash Buffer NT3 Concentrate

Removes contamination from DNA that has been bound to the column membrane. Macherey-Nagel™ Wash Buffer NT3 Concentrate provides additional wash buffer for applications requiring larger buffer volumes or when the supply of wash buffer needs to be replenished. BUFFER NT3 20ML CONCENTRATE TO MAKE 100MLbuffer Macherey-Nagel 100mL

Macherey Nagel Bioanalysis™ NucleoSpin™ RNA Plus Isolation Column

For use in RNA isolation from cultured cells, animal tissue, plant tissue, microorganisms, RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays. Macherey Nagel Bioanalysis NucleoSpin™ RNA Plus renders lysate clearin Nucleospin RNA plus 50 preps

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