Thermo Scientific™ Pierce™ NHS-Phosphine Staudinger Ligation Reagents

Derivatize proteins or amine surfaces for selective conjugation, detection or purification of azide-tagged targets with these activated phosphine compounds.

Brand: Thermo Scientific™

Manufacturer Part Number: 88900

UNSPSC: 12161502

Code: PN

Additional Details:
Additional Details: Weight: 0.22680kg

Product Code. 10525104

Quantity Price
1 £ 283.0 / 10mg
Estimated Shipment date
from Supplier 09-05-2017
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Description and Specification


Formulation Phosphine functionalized as N-hydroxysuccinimide ester; powder solid
Cleavable No
Molecular Weight 461.4
Sufficient For 22mL of typical 1X (1mM) reaction solution, or approx. 5 uses at 2mg reagent per use
Spacer Arm Length 5.4Å
Description Pierce NHS-Phosphine
Membrane Permeable Yes
Chemical Reactivity Amine-to-Nonselective
PEGylated No
Water Soluble No
Quantity 10mg

These NHS-ester compounds react to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The phosphine groups (PPh2) react with azide-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. In combination with different azide-tagged compounds, these NHS-Phosphine reagents facilitate a variety of labeling and crosslinking experimental strategies for investigating protein interactions and cellular pathways.


  • Versatile – Sulfo-NHS-Phosphine easily dissolves in water-miscible solvents for subsequent dilution in aqueous reaction mixtures for azide conjugation; NHS-Phosphine can be used for amine surface modification in organic solvents

  • Compatible – reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents

  • Specific – NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)

  • Chemoselective – azide and phosphine groups do not react or interfere with components of biological samples but conjugate to one another with high efficiency