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Thermo Scientific™ NHS-Phosphine

Product Code. 10525104 Shop All Thermo Scientific Products
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Quantity:
10 mg
Unit Size:
10mg
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Product Code. 10525104

Brand: Thermo Scientific™ 88900

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This item is not returnable. View return policy

Description

Derivatize proteins or amine surfaces for selective conjugation, detection or purification of azide-tagged targets with these activated phosphine compounds.

Thermo Scientific™ Pierce NHS-Phosphine and Sulfo-NHS-Phosphine are amine-reactive compounds that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to azide-tagged molecules.

These NHS-ester compounds react to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The phosphine groups (PPh2) react with azide-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. In combination with different azide-tagged compounds, these NHS-Phosphine reagents facilitate a variety of labeling and crosslinking experimental strategies for investigating protein interactions and cellular pathways.

Highlights:

  • Versatile – Sulfo-NHS-Phosphine easily dissolves in water-miscible solvents for subsequent dilution in aqueous reaction mixtures for azide conjugation; NHS-Phosphine can be used for amine surface modification in organic solvents

  • Compatible – reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents

  • Specific – NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)

  • Chemoselective – azide and phosphine groups do not react or interfere with components of biological samples but conjugate to one another with high efficiency

TRUSTED_SUSTAINABILITY

Specifications

Chemical Reactivity Amine-Azide
Cleavable No
Description NHS-Phosphine
Molecular Weight (g/mol) 461.4
PEGylated No
Spacer Arm Length 5.4 Å
Content And Storage Upon receipt store at -20°C protected from light and moisture.
Cell Permeability Not determined
Shipping Condition Ambient
Product Line Pierce
Labeling Method Chemical Labeling
Crosslinker Type Heterobifunctional
Reactive Moiety NHS Ester, Phosphine
Spacer Short (<10 Å)
Form Powder
Quantity 10 mg
Solubility DMF, DMSO
Format Standard
Water Soluble No
Product Type Crosslinker
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For Research Use Only. Not for use in diagnostic procedures.

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