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Thermo Scientific™ Pierce™ NHS-Diazirine Crosslinkers

Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).

Brand:  Thermo Scientific™ 26167

Code : PN

Additional Details : CAS Number : 317854-65-2 Weight : 0.45360kg

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Product Code. 11891415

  • £192.00 / 50mg


Estimated Shipment
27-11-2017
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Description

Description

The succinimidyl-ester diazirine (SDA) reagents are a new class of crosslinkers that combine proven amine-reactive chemistry with an innovative and efficient diazirine-based photochemistry for conjugating amine-containing molecules to nearly any other functional group. The SDA crosslinkers include six compounds differing in spacer arm lengths, ability to cleave the crosslinked proteins, and presence or absence of a charged group for membrane permeability. Protein crosslinking is an important technique used to understand protein structure and to stabilize protein-protein interactions. Thermo Scientific SDA Reagents extend the efficiency and range of interactions that can be explored by this approach.

Highlights:

  • Versatile - different amine-reactive diazirine derivatives capture specific intracellular or cell-surface protein interactions

  • Controllable - two-step chemical crosslinking is activated using common laboratory UV lamps

  • Reversible - disulfide-containing spacers enable cleavage using reducing agents

  • Easy - reagents are photo-stable under typical laboratory lighting conditions so there is no need to perform experiments in the dark

  • Better than aryl azides - diazirine photo-reactive group has better photostability and is more efficiently activated by long-wave UV light

Specifications

Specifications

Amine-to-Nonselective
Pierce SDA
Yes
No
222mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
No
No
Succinimidyl 4,4'-azipentanoate; solid
225.2
50mg
3.9Å
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