Thermo Scientific™ Pierce™ NHS-Diazirine Crosslinkers

Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 26167

NHS-DIAZIRINE(SDA)50MG

UNSPSC: 12161502

Code: PN

Additional Details:
Additional Details: CAS Number: 317854-65-2 Weight: 0.45360kg



Product Code. 11891415

Quantity Price
1 £ 192.0 / 50mg
EU Stock 4
Add to basket


Description and Specification

Specification

Chemical Reactivity Amine-to-Nonselective
Cleavable No
Description Pierce SDA
Formulation Succinimidyl 4,4'-azipentanoate; solid
Membrane Permeable Yes
Molecular Weight 225.2
PEGylated No
Quantity 50mg
Sufficient For 222mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
Spacer Arm Length 3.9Å
Water Soluble No

The succinimidyl-ester diazirine (SDA) reagents are a new class of crosslinkers that combine proven amine-reactive chemistry with an innovative and efficient diazirine-based photochemistry for conjugating amine-containing molecules to nearly any other functional group. The SDA crosslinkers include six compounds differing in spacer arm lengths, ability to cleave the crosslinked proteins, and presence or absence of a charged group for membrane permeability. Protein crosslinking is an important technique used to understand protein structure and to stabilize protein-protein interactions. Thermo Scientific SDA Reagents extend the efficiency and range of interactions that can be explored by this approach.

Highlights:

  • Versatile - different amine-reactive diazirine derivatives capture specific intracellular or cell-surface protein interactions

  • Controllable - two-step chemical crosslinking is activated using common laboratory UV lamps

  • Reversible - disulfide-containing spacers enable cleavage using reducing agents

  • Easy - reagents are photo-stable under typical laboratory lighting conditions so there is no need to perform experiments in the dark

  • Better than aryl azides - diazirine photo-reactive group has better photostability and is more efficiently activated by long-wave UV light

Documentation

Technical documents