Thermo Scientific™ Pierce™ NHS-Diazirine Crosslinkers

Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).

Brand: Thermo Scientific™

Manufacturer Part Number: 26175

UNSPSC: 12161502

Code: PN

Additional Details:
Additional Details: CAS Number: 342405-30-5 Weight: 1.00000kg

Product Code. 10698864

Quantity Price
1 £ 297.0 / 50mg
EU Stock 1
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Description and Specification


Formulation Sulfosuccinimidyl 2-([4,4'-azipentanamido]ethyl)-1,3'-dithiopropionate; solid
Cleavable Yes
Sufficient For 102mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
Molecular Weight 490.51
Spacer Arm Length 13.5Å
Membrane Permeable No
Description Pierce Sulfo-SDAD
PEGylated No
Chemical Reactivity Amine-to-Nonselective
Water Soluble Yes
Quantity 50mg

The succinimidyl-ester diazirine (SDA) reagents are a new class of crosslinkers that combine proven amine-reactive chemistry with an innovative and efficient diazirine-based photochemistry for conjugating amine-containing molecules to nearly any other functional group. The SDA crosslinkers include six compounds differing in spacer arm lengths, ability to cleave the crosslinked proteins, and presence or absence of a charged group for membrane permeability. Protein crosslinking is an important technique used to understand protein structure and to stabilize protein-protein interactions. Thermo Scientific SDA Reagents extend the efficiency and range of interactions that can be explored by this approach.


  • Versatile - different amine-reactive diazirine derivatives capture specific intracellular or cell-surface protein interactions

  • Controllable - two-step chemical crosslinking is activated using common laboratory UV lamps

  • Reversible - disulfide-containing spacers enable cleavage using reducing agents

  • Easy - reagents are photo-stable under typical laboratory lighting conditions so there is no need to perform experiments in the dark

  • Better than aryl azides - diazirine photo-reactive group has better photostability and is more efficiently activated by long-wave UV light


Technical documents