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Thermo Scientific™ Pierce™ NHS-Diazirine Crosslinkers

Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).

Brand:  Thermo Scientific™ 26175

Code : PN

Additional Details : CAS Number : 342405-30-5 Weight : 1.00000kg

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Product Code. 10698864

  • £297.00 / 50mg


Estimated Shipment
07-12-2017
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Description

Description

The succinimidyl-ester diazirine (SDA) reagents are a new class of crosslinkers that combine proven amine-reactive chemistry with an innovative and efficient diazirine-based photochemistry for conjugating amine-containing molecules to nearly any other functional group. The SDA crosslinkers include six compounds differing in spacer arm lengths, ability to cleave the crosslinked proteins, and presence or absence of a charged group for membrane permeability. Protein crosslinking is an important technique used to understand protein structure and to stabilize protein-protein interactions. Thermo Scientific SDA Reagents extend the efficiency and range of interactions that can be explored by this approach.

Highlights:

  • Versatile - different amine-reactive diazirine derivatives capture specific intracellular or cell-surface protein interactions

  • Controllable - two-step chemical crosslinking is activated using common laboratory UV lamps

  • Reversible - disulfide-containing spacers enable cleavage using reducing agents

  • Easy - reagents are photo-stable under typical laboratory lighting conditions so there is no need to perform experiments in the dark

  • Better than aryl azides - diazirine photo-reactive group has better photostability and is more efficiently activated by long-wave UV light

Specifications

Specifications

Amine-to-Nonselective
Pierce Sulfo-SDAD
No
No
102mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
Yes
Yes
Sulfosuccinimidyl 2-([4,4'-azipentanamido]ethyl)-1,3'-dithiopropionate; solid
490.51
50mg
13.5Å
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