Thermo Scientific™ Pierce™ NHS-Diazirine Crosslinkers
Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).
Brand: Thermo Scientific™ 26173
Code : PN
Additional Details : CAS Number : 79455-62-2 Weight : 1.00000kg Transport : UN number : 3276 Chem class : 1 Pack group : III
DescriptionThe succinimidyl-ester diazirine (SDA) reagents are a new class of crosslinkers that combine proven amine-reactive chemistry with an innovative and efficient diazirine-based photochemistry for conjugating amine-containing molecules to nearly any other functional group. The SDA crosslinkers include six compounds differing in spacer arm lengths, ability to cleave the crosslinked proteins, and presence or absence of a charged group for membrane permeability. Protein crosslinking is an important technique used to understand protein structure and to stabilize protein-protein interactions. Thermo Scientific SDA Reagents extend the efficiency and range of interactions that can be explored by this approach.
Versatile - different amine-reactive diazirine derivatives capture specific intracellular or cell-surface protein interactions
Controllable - two-step chemical crosslinking is activated using common laboratory UV lamps
Reversible - disulfide-containing spacers enable cleavage using reducing agents
Easy - reagents are photo-stable under typical laboratory lighting conditions so there is no need to perform experiments in the dark
Better than aryl azides - diazirine photo-reactive group has better photostability and is more efficiently activated by long-wave UV light
|153mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use|
|Sulfosuccinimidyl 4,4'-azipentanoate; solid|