Thermo Scientific™ BS3-d4 (bis(sulfosuccinimidyl) 2,2,7,7-suberate-d4)

Product Code. 10658674

Brand: Thermo Scientific™ 21595

Description

Crosslink amines to amines using these heavy (deuterated) and light matched pairs of an 11.4A, NHS-ester crosslinker for mass spectrometry analysis of protein interactions.

Thermo Scientific™ Pierce BS3-d0/d4 reagents form a matched set of heavy (deuterated) and light mass varieties of the popular BS3 amine-reactive crosslinker that facilitates the identification of crosslinked peptides by mass spectrometry.

A new strategy to study protein structure and function is rapidly emerging that integrates the proven utility of crosslinking with the power of mass spectrometry (MS) to yield insights into protein tertiary structure and protein complex formation. The reagents introduced here will generate MS patterns in which the resultant crosslinked peptides will differ by 4 mass units after enzymatic digestion of the crosslinked protein or protein complex.

Highlights:

• Well-characterized, high-purity, deuterium-labeled crosslinker and its hydrogen-containing analog
• Instructions edited by an expert in the crosslinking/MS strategy field
• Suberate- and glutarate-based reagent pair - provides a “molecular ruler” option to study inter- and intra-molecular distances to gain structural information not possible with just one reagent
• Efficient and convenient - requires only microgram amounts of protein; an excellent alternative to NMR or X-ray crystallography-based methods that require large amounts of protein, special solvents or crystal formation
• Reactive groups: Sulfo-NHS ester (homobifunctional)
• Reactive toward: amino groups

Note:

References Illustrating Applications of Heavy/Light Crosslinking Reagents and Mass Spectrometry Sinz, A. (2003). Chemical crosslinking and mass spectrometry for mapping three-dimensional structures of proteins and protein complexes.J. Mass Spectrom.38, 1225-1237. Dihazi, G.H. and Sinz, A. (2003). Mapping low-resolution three-dimensional protein structure using chemical crosslinking and Fourier transform ion-cyclotron resonance mass spectrometry. Rapid Commun. Mass Spectrom.17, 2005-2014. Kalkhof, S.et al.(2005).

Specifications

• Chemical Reactivity: Amine-Amine
• Cleavable: No
• Description: BS3-d4
• Molecular Weight (g/mol): 576.45
• PEGylated: No
• Spacer Arm Length: 11.4 Å
• Content And Storage: Upon receipt store desiccated at 4 to 8°C.
• Cell Permeability: No
• Shipping Condition: Ambient
• Product Line: Pierce™
• Labeling Method: Chemical Labeling
• Crosslinker Type: Homobifunctional
• Reactive Moiety: Sulfo-NHS Ester
• Spacer: Medium (10 to 30 Å)
• Form: Powder
• Quantity: 10 mg
• Solubility: Water
• Format: Standard
• Water Soluble: Yes
• Product Type: Crosslinker

Safety and Handling

Product Identifier

• BS3-d4 (bis(sulfosuccinimidyl) 2,2,7,7-suberate-d4)

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P271-Use only outdoors or in a well-ventilated area.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P403+P233-Store in a well-ventilated place. Keep container tightly closed.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : bis(sulfosuccinimidyl) 2,2,7,7-suberate-d4

For Research Use Only. Not for use in diagnostic procedures.