Thermo Scientific™ Pierce™ ChIP-Grade Protein A/G Plus Agarose

Chromatin immunoprecipitation (chromatin IP, ChIP) assay kit containing all reagents, agarose affinity beads, and a simple, fast, reliable protocol.

Brand:  Thermo Scientific™ 26159

Product Code. 10586985

Description

Thermo Scientific™ Pierce Agarose ChIP Kit provides a complete set of reagents and a simple, fast and reproducible protocol to perform chromatin immunoprecipitation (ChIP) assays.

This chromatin IP kit contains sufficient reagents to perform 30 ChIP assays with appropriate controls using an optimized protocol and a convenient spin-column format. The kit includes a complete Chromatin Prep Module (also available separately) to efficiently extract and solubilize crosslinked DNA-protein interaction complexes from cells, plus an immunoprecipitation module that contains high-capacity, ChIP-grade Protein A/G agarose.

Highlights:

• Simple and streamlined ChIP assay protocol (< 8 hours)
• Highly efficient isolation and lysis of nuclei
• Easy and reproducible enzymatic digestion
• Low-background and high-capacity Protein A/G agarose resin
• Highly specific positive control reagents included: RNA polymerase II antibody and GAPDH PCR primers
• Fast and reproducible spin-column format; columns and collection tubes included
• High-recovery DNA purification
• Choose a complete ChIP assay kit or the separate chromatin preparation module

Includes:

Kit contains the Chromatin Prep Module (Part No. 26158; 11 components) for chromatin extraction and a complete set of immunoprecipitation reagents (15 components) for IP experiments with controls

Specifications

• Binding Capacity: >50 mg Human IgG/mL resin binding capacity
• Description: Pierce ChIP-Grade Protein A/G Plus Agarose
• Form: Liquid Suspension
• Quantity: 0.65 mL
• Type: Agarose
• Format: Bottle

• Capacity (Metric): ≥50 mg Human IgG per mL Resin Binding
• Content And Storage: Upon receipt store at 4°C
• Product Line: Pierce™
• Target: IgG Antibody
• Stationary Phase: Protein A/G
• Column Type: Affinity, Agarose Resin

For Research Use Only. Not for use in diagnostic procedures.