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Protein Purification Reagents and Kits

A variety of reagents, columns, kits, and associated products designed for high-yield purification of proteins.
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1

Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

EDGE
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
2
Promotions Available

Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

EDGE
Stationary Phase Divalent Nickel, Metal Chelate
Type Resin
Target His-Tagged Protein
Content And Storage Store at 4°C
Format Bottle
Description HisPur Ni-NTA Resin
Form Liquid Suspension
Column Type Affinity, Agarose Resin
Capacity (Metric) Up to 60 mg of 6xHis-Tagged Protein Binding per mL Resin
Product Line HisPur
3

Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

EDGE
Flow Rate <20 mL/min.
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.6 in.
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Diameter (Metric) Inner 16 mm
Material Polypropylene
Support Type 6% Cross-linked Agarose
4

Cytiva His GraviTrap™ Columns

Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.

EDGE
5

Cytiva PD MiniTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

EDGE
6

Cytiva StrepTrap XT Chromatography Column

StrepTrap XP is a column packed with Strep-Tactin™ XT Sepharose resin to purify Strep-tag™ II and Twin-Strep-tag™ recombinant proteins.

EDGE
Height (Metric) 25 mm
Column Type Affinity Column
For Use With (Application) Purification of recombinant proteins tagged with Strep-tag™ II and Twin-Strep-tag™
7
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Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose

Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Stationary Phase Divalent Nickel, Metal Chelate
Type Agarose
Target His-Tagged Protein
Content And Storage Store at 4°C
Format Bottle
Form Liquid Suspension
Column Type Affinity, Agarose Resin
Capacity (Metric) 20 mg His-Tagged GFP Binding per mL Resin
Product Line HisPur
8
Promotions Available

Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells

Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.

EDGE
9

Thermo Scientific™ HisPur™ Ni-NTA Chromatography Cartridges, 1 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

EDGE
Stationary Phase Divalent Nickel, Metal Chelate
Type Chromatography Cartridge
Target His-Tagged Protein
Content And Storage Store between 4°C and 8°C. Do not freeze.
Column Type Affinity, Agarose Resin
Product Line HisPur
10

Cytiva HisTrap™ excel Prepacked Columns

For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.

EDGE
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 2 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
11
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Cytiva HiScreen™ Capto™ Q ImpRes and Capto™ SP ImpRes

High-resolution intermediate purification and polishing based on the well-established Capto platform with traditional ligands

EDGE
Particle Size 36 to 44μm
Buffer 1M NaOH, 8M Urea, 6M Guanidine Hydrochloride, 30% Isopropanol and 70% Ethanol
Flow Rate 220 cm/hr.
Height (Metric) 200mm
Content And Storage 4° to 30°C
Diameter (English) Inner 39.3 in.
Diameter (Metric) Inner 1000mm
dimMatrix Capto Q Chromatography Media
Quantity 100 mL
For Use With (Application) High-throughput intermediate purification and polishing steps of a wide range of biomolecules
Support Type High-flow Agarose
Max. Pressure <3 bar
Height (English) 7.87 in.
12

IBA Lifesciences 10x Buffer BXT

IBA Lifesciences GmbH 10x Buffer BXT; Strep-Tactin™XT Elution B. is a ready-to-use buffer for elution of Strep-tagged proteins from Strep-Tactin™ XT.

EDGE
13
Promotions Available

Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 0.2 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

14

IBA Lifesciences Strep-Tactin™XT 4Flow™ High Capacity Resin
BIOPHARMA

Strep-Tactin™XT 4Flow™ high capacity resin is a 50% suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

EDGE
15

Invitrogen™ ProBond™ Nickel-Chelating Resin

A nickel-charged affinity resin used to purify recombinant proteins containing a polyhistidine (6xHis) sequence.

Type Resin
Stationary Phase Nickel-Chelating
Content And Storage The ProBond™ resin is pre-charged and able to bind 1-5 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 20% ethanol. The resin will appear blue in color when charged with Ni2+ . Store at +4°C. ProBond™ resin is guaranteed stable for 6 months when properly stored.
Form Suspension
Product Line ProBond