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Invitrogen™ Mouse TNF alpha, Recombinant Protein
Recombinant Protein
£61.00 - £130.00
Specifications
Molecular Weight (g/mol) | The protein is not methionylated at the N-terminal and has a predicted molecular mass of 17,258. The DTT-reduced and non-reduced protein migrate as 17kDa polypeptides on SDS-PAGE. |
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Purification Method | SDS-PAGE |
Source | E. coli |
Regulatory Status | RUO |
Structural Form | E. coli expressed amino acids Leu 80-Leu 235 of mouse TNF- alpha accession # NM_013693 |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15587516
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Affymetrix eBioscience
14-8321-62 |
5μg |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
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15255323
|
Affymetrix eBioscience
14-8321-63 |
10μg |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
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Description
Tumor Necrosis Factor-Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor-bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single ∽17kDa band following SDS-PAGE under non-reducing conditions. Description: Mouse TNF-a is a 17kDa factor produced by macrophages, monocytes, neutrophils, CD4^+ T cells and NK cells. A 26kDa form of TNF-alpha is expressed as a membrane-bound molecule. TNF-alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF-alpha have been observed. Applications Reported: Recombinant mouse TNF alpha is biologically active and can induce cytolysis of the L929 mouse cell line in culture. Applications Tested: The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25 pg/mL. This corresponds to a specific activity of greater than or equal to 4 x 10e7 Units/mg. Source: E. coli expressed amino acids Leu 80-Leu 235 of mouse TNF-alpha accession # NM_013693. Bioactivity: The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25 pg/mL. This corresponds to a specific activity of greater than or equal to 4 x 10e7 Units/mg. Endotoxin: Less than 0.01ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: The protein is not methionylated at the N-terminal and has a predicted molecular mass of 17,258. The DTT-reduced and non-reduced protein migrate as 17kDa polypeptides on SDS-PAGE. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2μm post-manufacturing filtered.Specifications
The protein is not methionylated at the N-terminal and has a predicted molecular mass of 17,258. The DTT-reduced and non-reduced protein migrate as 17kDa polypeptides on SDS-PAGE. | |
E. coli | |
E. coli expressed amino acids Leu 80-Leu 235 of mouse TNF- alpha accession # NM_013693 | |
The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25pg/mL. This corresponds to a specific activity of greater than or equal to 4 x 107 Units/mg. | |
Recombinant |
SDS-PAGE | |
RUO | |
Less than 0.01ng/μg cytokine as determined by the LAL assay. | |
Unconjugated |
For Research Use Only.