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Invitrogen™ Mouse TNF alpha, Recombinant Protein
Recombinant Protein
Brand: Affymetrix eBioscience 14-8321-63
Code : NEW
Additional Details : Weight : 0.10000kg
Description
Tumor Necrosis Factor-Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor-bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single ∽17kDa band following SDS-PAGE under non-reducing conditions. Description: Mouse TNF-a is a 17kDa factor produced by macrophages, monocytes, neutrophils, CD4^+ T cells and NK cells. A 26kDa form of TNF-alpha is expressed as a membrane-bound molecule. TNF-alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF-alpha have been observed. Applications Reported: Recombinant mouse TNF alpha is biologically active and can induce cytolysis of the L929 mouse cell line in culture. Applications Tested: The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25 pg/mL. This corresponds to a specific activity of greater than or equal to 4 x 10e7 Units/mg. Source: E. coli expressed amino acids Leu 80-Leu 235 of mouse TNF-alpha accession # NM_013693. Bioactivity: The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25 pg/mL. This corresponds to a specific activity of greater than or equal to 4 x 10e7 Units/mg. Endotoxin: Less than 0.01ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: The protein is not methionylated at the N-terminal and has a predicted molecular mass of 17,258. The DTT-reduced and non-reduced protein migrate as 17kDa polypeptides on SDS-PAGE. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2μm post-manufacturing filtered.Specifications
P06804 | |
Control, Functional Assay | |
21926 | |
Mouse TNF alpha | |
SDS-PAGE | |
E. coli | |
RUO | |
Less than 0.01ng/μg cytokine as determined by the LAL assay. | |
Tnf | |
Mouse | |
Ms | |
Recombinant | |
Recombinant |
0.1mg/mL | |
PBS with no preservative; pH 7.2 | |
The protein is not methionylated at the N-terminal and has a predicted molecular mass of 17,258. The DTT-reduced and non-reduced protein migrate as 17kDa polypeptides on SDS-PAGE. | |
7.2 | |
10μg | |
-80°C | |
E. coli expressed amino acids Leu 80-Leu 235 of mouse TNF- alpha accession # NM_013693 | |
Mouse TNF alpha | |
The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25pg/mL. This corresponds to a specific activity of greater than or equal to 4 x 107 Units/mg. | |
Unconjugated | |
E. coli | |
Liquid | |
Affinity chromatography |
For Research Use Only.