Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

Product Code	Brand	Quantity
10126414	Cytiva 17-5319-01	5 x 1 mL
11595674	Cytiva 17-5319-02	100 x 1 mL

Description

• Prepacked with precharged Ni Sepharose™ 6 Fast Flow media
• Column material: polypropylene
• Bed dimensions: 16 × 25mm
• Flow velocity: <20mL/min.
• Protein binding capacity of ∽40mg/mL media with negligible nickel ion leakage
• Compatible with wide range of reducing agents, detergents, denaturants, and other additives
• Simple operation with syringe, perstaltic pump, or chromatographic system
• Columns may be connected in series to increase capacity

Medium

• Matrix: 6% cross-linked agarose
• Average particle size: 90μm
• pH stability working range: 3 to 12

Specifications

• Diameter (English) Inner: 0.6 in.
• Description: HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
• Material: Polypropylene
• Support Type: 6% Cross-linked Agarose
• Max. Operating Pressure: 5 bar (0.5 MPa, 70 psi)

• Diameter (Metric) Inner: 16 mm
• Flow Rate: <20 mL/min.
• pH Range: 3 to 12
• Content And Storage: 4°C to 30°C, 20% Ethanol
• Type: Pre-packed Column

Safety and Handling

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.
• EUH208-Contains nickel. May produce an allergic reaction.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
• P405-Store locked up.

Supplemental information

• MIXTURE LIST-Contains : ethanol, Nickel