PCR Reagents and Kits

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

Nuclease-free Water (not DEPC-treated) has been deionized, filtered into the final bottle, and autoclaved. It is ready to use and requires no preparation, mixing, or autoclaving.

Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase (2 U/μL)

Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.

Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR

Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR

TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms. It provides best in class performance including the widest quantifiable range, in both single and duplex reactions, even with challenging targets.

Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Invitrogen™ EDTA (0.5 M), pH 8.0, RNase-free

Invitrogen™ EDTA (0.5 M), pH 8.0, RNase-free

Invitrogen Molecular biology grade, 0.5 M EDTA, pH 8.0 solution is supplied in one bottle. The solution is certified RNase-free, economical, and ready-to-use.

Applied Biosystems™ TaqMan™ Universal PCR Master Mix

Applied Biosystems™ TaqMan™ Universal PCR Master Mix

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.

Invitrogen™ MgCl<sub>2</sub> (1 M)

Invitrogen™ MgCl2 (1 M)

Molecular biology grade, Invitrogen 1M MgCl2 solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use.

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) is used in the purification of nucleic acids. This reagent consists of highly pure chloroform, isoamyl alcohol, and UltraPure™ Phenol saturated with Tris-HCl.

TaqMan™ TaqMan™ Gene Expression Master Mix

TaqMan™ TaqMan™ Gene Expression Master Mix

Precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems™ TaqMan™ Gene Expression Master Mix.

Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR

Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR

TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms.

Applied Biosystems™ SYBR™ Green Universal Master Mix

Applied Biosystems™ SYBR™ Green Universal Master Mix

Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.

Cytiva PuReTaq Ready-To-Go™ PCR Beads

Cytiva PuReTaq Ready-To-Go™ PCR Beads

Pre-mixed, pre-dispensed reaction beads optimized for performing standard PCR amplifications.  Cytiva Lifescience™ illustra™ PuReTaq Ready-To-Go™ PCR Beads are stable at ambient temperature.

Invitrogen™ GlycoBlue™ Coprecipitant (15 mg/mL)

Invitrogen™ GlycoBlue™ Coprecipitant (15 mg/mL)

GlycoBlue Coprecipitant consists of a blue dye covalently linked to glycogen, a branched chain carbohydrate, which is useful as a nucleic acid coprecipitant.

TaqMan® TaqMan™ Gene Expression Assay (FAM)

TaqMan® TaqMan™ Gene Expression Assay (FAM)

Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5' end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3' end.

Invitrogen™ Recombinant Proteinase K Solution (20 mg/mL)

Invitrogen™ Recombinant Proteinase K Solution (20 mg/mL)

Recombinant Proteinase K is a nonspecific serine protease that is useful for general digestion of proteins.

Invitrogen™ DEPC-Treated Water

Invitrogen™ DEPC-Treated Water

Ambion DEPC-treated water is certified nuclease-free. DEPC-treated water is autoclaved pre- and post-packaging to ensure sterility and inactivation of DEPC.

SYBR™ <i>Power</i> SYBR™ Green PCR Master Mix

SYBR™ Power SYBR™ Green PCR Master Mix

Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.

Invitrogen™ PureLink™ RNase A (20 mg/mL)

Invitrogen™ PureLink™ RNase A (20 mg/mL)

PureLink™ RNase A (ribonuclease A) is a bovine pancreatic ribonuclease that cleaves single-stranded RNA.

Invitrogen™ KCl (2 M), RNase-free

Invitrogen™ KCl (2 M), RNase-free

Invitrogen molecular biology grade, 2M KCl solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use.

Invitrogen™ RT-PCR Grade Water

Invitrogen™ RT-PCR Grade Water

RT-PCR Grade Water is certified free of nucleases, and free of nucleic acid contamination that may cause false-positive signals in RT-PCR. The RT-PCR Grade Water is ready to use and requires no preparation, mixing, or autoclaving.

Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF Buffer

Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF Buffer

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.

Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix

Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix

Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.

Invitrogen™ UltraPure™ BSA (50 mg/mL)

Invitrogen™ UltraPure™ BSA (50 mg/mL)

Ambion Ultrapure BSA is a 'non-acetylated' BSA pure enough to use when DNA or RNA integrity is critical.

Invitrogen™ <i>Taq</i> DNA Polymerase, recombinant

Invitrogen™ Taq DNA Polymerase, recombinant

A thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer

SYBR™ Fast SYBR™ Green Master Mix

SYBR™ Fast SYBR™ Green Master Mix

Designed for speed, SYBR™ Green Master Mix provides a fast, reliable, and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range, or PCR efficiency.

Invitrogen™ Proteinase K Solution (20 mg/mL), RNA grade

Invitrogen™ Proteinase K Solution (20 mg/mL), RNA grade

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.

Invitrogen™ Sodium Acetate (3 M), pH 5.5, RNase-free

Invitrogen™ Sodium Acetate (3 M), pH 5.5, RNase-free

Invitrogen Molecular biology grade, 3M Sodium Acetate solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use.

Invitrogen™ Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1)

Invitrogen™ Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1)

In RNA extraction procedures, Acid Phenol:Chloroform:IAA aids in the removal of DNA (it partitions into the organic phase), helps to stabilize the interface, and prevents foaming when mixing.

Thermo Scientific™ Phusion Green High-Fidelity DNA Polymerase (2 U/μL)

Thermo Scientific™ Phusion Green High-Fidelity DNA Polymerase (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.

Maxima Maxima SYBR Green/ROX qPCR Master Mix (2X)

Maxima Maxima SYBR Green/ROX qPCR Master Mix (2X)

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

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