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PowerUp™, SYBR™ PowerUp™ SYBR™ Green Master Mix

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

£111.00 - £2110.00

Specifications

Detection Method Detection Method: SYBR; PCR Method: qPCR
Sample Type DNA
Storage Requirements Store at 2° to 8°C
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Products 8
Product Code Brand No. of Reactions Quantity Price Quantity & Availability  
Product Code Brand No. of Reactions Quantity Price Quantity & Availability  
15350929 View Documents Applied Biosystems™
A25742
500 reactions 5mL £262.00
5mL View alternative products
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EU Stock 955 Please to purchase this item. Need a web account? Register with us today!
15360929 View Documents Applied Biosystems™
A25743
5000 reactions 50mL £2095.00
50mL View alternative products
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EU Stock 12 Please to purchase this item. Need a web account? Register with us today!
15310939 View Documents Applied Biosystems™
A25778
5000 reactions 10 x 5mL £2110.00
Pack of 10 View alternative products
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EU Stock 95 Please to purchase this item. Need a web account? Register with us today!
15330939 View Documents Applied Biosystems™
A25780
500 reactions 5 x 1mL £268.00
Pack of 5 View alternative products
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EU Stock 94 Please to purchase this item. Need a web account? Register with us today!
15340939 View Documents Applied Biosystems™
A25918
1000 reactions 10 x 1mL £496.00
Pack of 10 View alternative products
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EU Stock 47 Please to purchase this item. Need a web account? Register with us today!
15380929 View Documents Applied Biosystems™
A25776
1000 reactions 2 x 5mL £485.00
Pack of 2 View alternative products
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EU Stock 477 Please to purchase this item. Need a web account? Register with us today!
15390929 View Documents Applied Biosystems™
A25777
2500 reactions 5 x 5mL £1114.00
Pack of 5 View alternative products
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EU Stock 191 Please to purchase this item. Need a web account? Register with us today!
15366158 View Documents Applied Biosystems™
A25779
200 reactions 2 x 1mL £111.00
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EU Stock 236 Please to purchase this item. Need a web account? Register with us today!
Description

Description

  • A dual hot-start mechanism for excellent specificity
  • Highly reproducible CTs over a broad dynamic range
  • Inclusion of UDG to help prevent carryover contamination
  • Stability of pre-assembled reactions for up to 72 hours
  • Compatibility with most real-time qPCR instruments

Exceptional specificity with dual hot-start mechanism:
  • Specificity is paramount in obtaining high-quality data in SYBR™ Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding
  • PowerUp™ SYBR™ Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures
  • In an experiment evaluating 24 different primer sets, PowerUp™ SYBR™ Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification

Tight reproducibility in CTs over a wide dynamic range:
  • PowerUp™ SYBR™ Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested
  • In addition, this master mix provides efficient amplification over 6 logs of sample input
  • Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR™ Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality

UDG included for carryover contamination control:
  • The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR™ Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives

Stability of pre-assembled reactions:
  • The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR™ Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data
  • This consistency allows users to have confidence and flexibility in the use of PowerUp™ SYBR™ Green Master Mix across numerous workflow scenarios1

Broad instrument compatibility:
  • PowerUp™ SYBR™ Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments2
  • It is also compatible with the Bio-Rad IQ™5 and CFX96™/CFX384™ systems, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems
  • For optimal results, recommended primer concentrations are 300 to 800nM

Order Info

Shipping Conditions: Wet Ice

Specifications

Specifications

Detection Method: SYBR; PCR Method: qPCR
DNA
Store at 2° to 8°C

For Research Use Only. Not for use in diagnostic procedures.