missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.
£81.75 - £2535.00
Specifications
Detection Method | SYBR |
---|---|
Format | Tube |
PCR Method | qPCR |
Polymerase | Dual-Lock Taq DNA Polymerase |
Reaction Speed | Fast or Standard |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15320929
|
Applied Biosystems™
A25741 |
1 mL |
£81.75
1mL |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
15350929
|
Applied Biosystems™
A25742 |
5 mL |
£315.00
5mL |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
15360929
|
Applied Biosystems™
A25743 |
50 mL |
£2515.00
50mL |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
15310939
|
Applied Biosystems™
A25778 |
10 x 5 mL |
£2535.00
Pack of 10 |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
15330939
|
Applied Biosystems™
A25780 |
5 x 1 mL |
£322.00
Pack of 5 |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
15340939
|
Applied Biosystems™
A25918 |
10 x 1 mL |
£598.00
Pack of 10 |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
15380929
|
Applied Biosystems™
A25776 |
2 x 5 mL |
£584.00
Pack of 2 |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
15390929
|
Applied Biosystems™
A25777 |
5 x 5 mL |
£1338.00
Pack of 5 |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
15366158
|
Applied Biosystems™
A25779 |
2 x 1 mL |
£134.00
Pack of 2 |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
Description
Coupled with user-supplied primer sets and template, PowerUp™ SYBR™ Green Master Mix is designed to amplify targets for accurate gene expression analysis.
- A dual hot-start mechanism for excellent specificity
- Highly reproducible CTs over a broad dynamic range
- Inclusion of UDG to help prevent carryover contamination
- Stability of pre-assembled reactions for up to 72 hours
- Compatibility with most real-time qPCR instruments
Exceptional specificity with dual hot-start mechanism:
- Specificity is paramount in obtaining high-quality data in SYBR™ Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding
- PowerUp™ SYBR™ Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures
- In an experiment evaluating 24 different primer sets, PowerUp™ SYBR™ Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification
Tight reproducibility in CTs over a wide dynamic range:
- PowerUp™ SYBR™ Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested
- In addition, this master mix provides efficient amplification over 6 logs of sample input
- Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR™ Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality
UDG included for carryover contamination control:
- The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR™ Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives
Stability of pre-assembled reactions:
- The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR™ Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data
- This consistency allows users to have confidence and flexibility in the use of PowerUp™ SYBR™ Green Master Mix across numerous workflow scenarios1
Broad instrument compatibility:
- PowerUp™ SYBR™ Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments2
- It is also compatible with the Bio-Rad IQ™5 and CFX96™/CFX384™ systems, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems
- For optimal results, recommended primer concentrations are 300 to 800nM
Order Info
Shipping Conditions: Wet Ice
Specifications
SYBR | |
qPCR | |
Fast or Standard | |
PowerUP™, SYBR™ | |
Wet Ice | |
2X |
Tube | |
Dual-Lock Taq DNA Polymerase | |
7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, StepOne™, StepOnePlus™, ViiA™ 7 System | |
Real Time PCR SYBR Master Mix | |
Gene Expression | |
DNA |
For Research Use Only. Not for use in diagnostic procedures.
Spot an opportunity for improvement?Share a Content Correction
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title