Invitrogen™ Taq DNA Polymerase, recombinant

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.

Includes: Taq DNA Polymerase (5U/μL); 10X PCR buffer (200mM Tris-HCl pH 8.4, 500mM KCl); Magnesium Chloride (50mM)

Product Code	Brand	No. of Reactions
10174762	Invitrogen™ 10342053	100 Reactions
10033382	Invitrogen™ 10342020	500 Reactions
11508626	Invitrogen™ 10342046	1500 Reactions
10063262	Invitrogen™ 10342178	5000 Reactions

Description

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5'→3' DNA polymerase activity and a 5'→3' exonuclease activity.

With our Taq DNA Polymerase, you get:

• Your choice of recombinant or native enzyme
• Amplification of PCR products up to 5 kb in size
• An enzyme that is licensed and qualified for PCR

Applications

Taq DNA Polymerase is appropriate for use in the amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing.

Source

Recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli.

Unit definition

One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min. at 74°C.

For superior PCR performance, we recommend DreamTaq DNA Polymerase.

Order Info

Shipping Condition: Approved for shipment on wet or dry ice

Specifications

• Format: Stand-alone enzyme
• PCR Method: qPCR, Standard PCR
• Reaction Speed: Standard
• Product Type: DNA Polymerase
• For Use With (Application): Standard PCR
• Fidelity (vs. Taq): 1X
• Overhang: 3'-A
• Size (Final Product): 5 kb or less

• GC-Rich PCR Performance: Low
• Polymerase: Taq DNA Polymerase
• Exonuclease Activity: 5' - 3'
• Shipping Condition: Dry Ice
• Concentration: 5 U/μL
• Hot Start: No
• Reaction Format: Separate Components
• Starting Material: DNA

For Research Use Only. Not for use in diagnostic procedures.