Crosslinking, Labelling and Protein Modification

Thermo Scientific™ Pierce™ Urea

Pure, crystalline urea (CH4N2O), CAS 57-13-6; denaturant with low UV-absorptivity for protein biology methods (elution, solubilization, refolding, storage). 1 KG UREA, BULL'S EYE 1 1KG STORE AT AMBIENTtemperature

Thermo Scientific™ Pierce™ EDC Crosslinker

Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker. EDC 25GM

Invitrogen™ DTT, 1M

DTT, 1M DTT, 1M 1 MLDTT is an essential component for kinase assays

Invitrogen™ eBioscience™ 7-AAD Viability Staining Solution

Ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. Can be used in combination with PE- and FITC-conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is that there is minimal spectral overlap between these emissions. 2ML 7-AAD Viability Staining Solution

GE Healthcare Cy3B NHS Ester Mono-Reactive CyDye

Combines exceptional photostability with bright and intense signals. GE Healthcare Cy3B NHS Ester Mono-Reactive CyDye provides targeted and specific labeling of amine residues on antibodies, peptides, or oligonucleotides in fluorescence polarization applications. CY3B NHS ESTER

Thermo Scientific™ Pierce™ 8M Guanidine·HCl Solution

Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods. 8M GUANIDINE-HCL, 200 MLambient temp

Thermo Scientific™ Pierce™ TCEP·HCl

Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3). TCEP·HCL 10GM

Thermo Scientific™ Pierce™ SATA

Short (2.8A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies. SATA, 50 MGStore in Freezer at -20C

Thermo Scientific™ Bond-Breaker™ Neutral pH TCEP Solution

Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers. BOND-BREAKER TCEP SOLUTION, NEUTRAL PH, 5 ML

Molecular Probes™ Alexa Fluor™ 488 Protein Labeling Kit

Alexa Fluor™ 488 Protein Labeling Kit PROTEIN LABEL PROBES KOMBO, USES ALEXA FLUOR(R)488 dye, Protein labelling kit for 3X reactions

Thermo Scientific™ Pierce™ BS3 (Sulfo-DSS) Crosslinker

Crosslink amines to amines with BS3; composed of sulfo-NHS-ester groups separated by an 11.4A, water-soluble spacer arm that is useful for cell-surface protein crosslinking. 1 GR BS3

Thermo Scientific™ Pierce™ SMCC Crosslinker

Crosslink amines to sulfhydryls with this mid-length crosslinker composed of NHS-ester and maleimide groups at ends of a stabilizing cyclohexane-containing, 8-atom spacer arm. SMCC, 50 MGne-1-carboxylate 50mg Store in Fridge at 4C

Thermo Scientific™ Pierce™ Sulfo-NHS Crosslinker

Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7. SULFO-NHS 5G

Molecular Probes™ Alexa Fluor™ 647 NHS Ester (Succinimidyl Ester)

Alexa Fluor™ 647 NHS Ester (Succinimidyl Ester) ALEXA FLUOR(R) 647 CARBOXThe isomer-free, amine-reactive Alexa Fluor 647

Thermo Scientific™ Pierce™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) No-Weigh™ Format

Thermo Scientific™ Pierce™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) No-Weigh™ Format is a water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium cyclohexane spacer arm. X10 SULFO-SMCC NO-WEIGH, 2MG

Thermo Scientific™ Pierce™ Methyl-PEG-NHS-Ester Reagents

Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units). 100MG METHYL-PEO8-NHS ESTER

GE Healthcare Cy5.5 NHS Ester Mono-Reactive CyDye

Use for highly accurate labeling of amine groups. GE Healthcare Cy5.5 NHS Ester Mono-Reactive CyDye contain derivitized CyDyes that carry only one reactive group on each dye molecule for accurate labeling of amine groups, for example on antibodies, peptides, or oligonucleotides. CY5.5 MONO NHS ESTER

Molecular Probes™ Alexa Fluor™ 647 Azide, Triethylammonium Salt

Alexa Fluor™ 647 azide, triethylammonium salt 500 UG ALEXA FLUOR 647 AZIDE, TRIETHYLAMMONIUMsalt 0.5 mg Store at -20°C

Thermo Scientific™ Pierce™ DTT (Dithiothreitol) No-Weigh™ Format

Thermo Scientific™ Pierce™ DTT (Dithiothreitol) No-Weigh™ Format is designed for convenient preparation of stocks that will be added to protein samples for effective reduction of disulfide bonds. 48 TESTS Pierce DTT No-Weigh

Thermo Scientific™ Pierce™ BS3 (bis(sulfosuccinimidyl)suberate) No-Weigh™ Format

Thermo Scientific™ Pierce™ BS3 (bis(sulfosuccinimidyl)suberate) No-Weigh™ Format is an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable. X10 BS3 No-Weigh Format 2MG

Molecular Probes™ Dapoxyl™ Carboxylic Acid, Succinimidyl Ester

Dapoxyl™ carboxylic acid, succinimidyl ester CARBOXYLIC ACI, DAPOXYL(R) STORE AT -20C

Thermo Scientific™ Pierce™ Immobilized Papain

Generate and purify Fab fragments from antibodies with cysteine-endopeptidase papain immobilized onto beaded agarose resin. 5 ML IMMOBILISED PAPAIN, AGAROSE 5ML STORE INFridge at 4C

Thermo Scientific™ Pierce™ FITC and Fluorescein Dyes and Labeling Kits

Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518). 1 SET FITC ANTIBODY LABELLING KIT

GE Healthcare Amersham™ CyDye™ Value Packs (mono-Reactive NHS Ester)

Use for highly accurate labeling of amine groups. GE Healthcare Amersham™ CyDye™ Value Packs (mono-Reactive NHS Ester) contain derivitized CyDyes that carry only one reactive group on each dye molecule for accurate labeling of amine groups, for example on antibodies, peptides, or oligonucleotides. 1 MG CY3.5 NHS ESTER (MONO-REACTIVE NHS ESTER)(25190143) 1mg Store in Fridge at 4C

Thermo Scientific™ Pierce™ 2-Mercaptoethanol

Pure, liquid (equals 14M), beta-mercaptoethanol (bME, 2BME, 2-ME, b-mer), CAS 60-24-2; thiol reducing agent for cleaving protein disulfide bonds (cystines cysteines). 2-MERCAPTOETHANOL 10ML

Molecular Probes™ Monochlorobimane (mBCI)

monochlorobimane (mBCI) MONOCHLOROBIMANE (MBCL)Monochloromobimane is essentially nonfluorescent

Thermo Scientific™ Pierce™ Sulfo-NHS-Acetate

Amine-reactive (NHS ester) compound to permanently block primary amines (e.g., protein lysine side chains) to prevent interactions or later conjugation. 100 MG SULFO-NHS-ACETATE

Thermo Scientific™ Pierce™ Immobilized TCEP Disulfide Reducing Gel

Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized. 5 ML IMMOBILISED TCEP DISULFIDE REDUCING GEL 5MLStore in fridge at 4C

Thermo Scientific™ Pierce™ BMOE Crosslinker

Crosslink sulfhydryls to sulfhydryls (cysteine residues in proteins or peptides) with this short crosslinker composed of maleimide groups and a 7-atom (8.0A) spacer arm. 50 MG BMOE 50MG STORE IN FRIDGE AT 4C

Molecular Probes™ Alexa Fluor™ 647 C2 Maleimide

Alexa Fluor™ 647 C2 Maleimide C2-MALEIMIDE, ALEXA FLUOR(R) 647 STORE AT -20C