Protein Biology
Invitrogen™ NuPAGE™ LDS Sample Buffer (4X)
Used to prepare protein samples for denaturing gel electrophoresis with NuPAGE™ Novex™ gels. Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X) contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.
Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)
Made with high-purity reagents and are strictly quality controlled
Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)
Made with high-purity reagents and are strictly quality controlled.
Cytiva Disposable PD-10 Desalting Columns
Use for desalting and buffer exchange, as well as removal of low-molecular weight compounds. Cytiva Life Sciences™ Disposable PD-10 Desalting Columns provide fast, high-recovery.
Invitrogen™ NuPAGE™ Antioxidant
Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis
Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)
Ready-to-use reagent to reduce protein samples for protein gel electrophoresis
Invitrogen™ NuPAGE™ Transfer Buffer (20X)
Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting
Thermo Scientific™ DNA Gel Loading Dye (6X)
Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.
Thermo Scientific™ RIPA Lysis and Extraction Buffer
Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.
Thermo Scientific™ Pierce™ LTQ Velos ESI Positive Ion Calibration Solution
Calibrate LTQ Velos, LTQ Orbitrap and other mass spectrometers using a mixture of highly purified ionizable molecules designed for positive mode calibration.
Invitrogen™ iBlot™ 2 Transfer Stacks, nitrocellulose, regular size
Used to transfer proteins with the iBlot 2 Gel Transfer Device
Thermo Scientific™ Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL
Perform high-throughput protein recovery (7K MWCO) and sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening, and assay development with ready-to-use Thermo Scientific Pierce Zeba desalting chromatography columns, plates, and cartridges.
Triton™ X-100 (Electrophoresis), Fisher BioReagents™
CAS: 9002-93-1 Molecular Formula: C16H26O2 Molecular Weight (g/mol): 250.38 MDL Number: MFCD00132505 InChI Key: JYCQQPHGFMYQCF-UHFFFAOYSA-N Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether PubChem CID: 5590 SMILES: CC(C)(C)CC(C)(C)C1=CC=C(OCCO)C=C1
Invitrogen™ Novex™ Tris-Glycine SDS Sample Buffer (2X)
Use with Novex Tris-Glycine Gels
Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions
Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.
Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent
Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity. Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.
Cytiva Pharmalyte™ Carrier Ampholytes
Use in Isoelectric focusing. Cytiva Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.
Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™
Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.38 MDL Number: MFCD00036175 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: SDS, Sodium Lauryl Sulfate PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium dodecyl sulfate SMILES: [Na+].CCCCCCCCCCCCOS([O-])(=O)=O
Thermo Scientific™ SnakeSkin™ Dialysis Tubing
Perform desalting and buffer exchange for 10 to 100 mL samples with this 3.5K, 7K, and 10K molecular weight cut-off (MWCO), ready-to-use form of traditional dialysis membrane tubing. Securely close dialysis tubing up to 50 mm flat diameter with the sturdy, non-floating tube SnakeSkin Dialysis Clips.
Thermo Scientific™ DTT (dithiothreitol)
Stabilize enzymes and other proteins which possess free sulfhydryl groups with DTT, shown to restore activity lost by oxidation of these groups in vitro.
Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls
Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.
Invitrogen™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X)
Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels
Invitrogen™ Magnetic Stand-96
The stand has clear polyacryl base in standard SBS 96-well microplate footprint and uses 24 extremely strong permanent magnets for fast, easy magnetic separation in little as 30 seconds.
Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)
Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels
Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate
Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.
Thermo Scientific™ Pierce™ ECL Western Blotting Substrate
Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.
Invitrogen™ iBlot™ 2 Transfer Stacks, nitrocellulose, mini
Used to transfer proteins with the iBlot 2 Gel Transfer Device
Thermo Scientific™ DNase I Solution (2500 U/mL)
Improve protein extraction efficiency with this glycosylated polypeptide that cleaves single- and double-stranded DNA in cell lysates.
Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent
Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent provides highly intense signal output, for detection of medium to very low protein levels.
Thermo Scientific™ Pierce™ Negative Ion Calibration Solution
Calibrate LTQ mass spectrometer instruments using a mixture of highly purified ionizable molecules designed for negative mode calibration.