Direct PCR Reagents and Kits
Direct PCR Reagents and Kits
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Filtered Search Results
Thermo Scientific™ Phire Plant Direct PCR Master Mix
Amplify DNA directly from plant samples without prior DNA purification with Phire Plant Direct PCR Master Mix
Polymerase | Phire Hot Start II DNA polymerase |
---|---|
GC-Rich PCR Performance | Medium |
Reaction Format | SuperMix or Master Mix |
Product Type | Direct PCR Master Mix |
Sample Type | Plant |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Starting Material | DNA |
For Use With (Application) | Genotyping |
PCR Method | Direct PCR |
Product Line | Phire |
Invitrogen™ PureLink™ PCR Purification Kit
The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.
Shipping Condition | Room Temperature |
---|---|
Format | Kit |
Sample Type | PCR Products |
Isolation Technology | Silica Spin Column |
Run Time | 10 min. |
Thermo Scientific™ GeneJET PCR Purification Kit
Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.
Format | Spin Column |
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Run Time | 5 min. |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Thermo Scientific™ Phire Tissue Direct PCR Master Mix
Amplify DNA directly from variety of human and animal samples with no prior purification with Phire Tissue Direct PCR Master Mix.
Polymerase | Phire Hot Start II DNA polymerase |
---|---|
GC-Rich PCR Performance | Medium |
Includes Label or Dye | Yes |
Reaction Format | SuperMix or Master Mix |
Product Type | Direct PCR Master Mix |
Sample Type | Tissue |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Starting Material | DNA |
For Use With (Application) | Genotyping |
PCR Method | Direct PCR |
Product Line | Phire |
Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit
The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.
For Use With (Equipment) | 7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ Chromo4, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
---|---|
Sample Type | Cells |
Reaction Speed | Fast or Standard |
Concentration | 2X |
For Use With (Application) | RT-PCR |
Product Line | CellsDirect™, Platinum™, SuperScript™ |
Detection Method | Primer-probe |
Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
GC-Rich PCR Performance | High |
---|---|
Format | Tube |
Product Type | 1-Step qPCR kit |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
Sample Type | Cell, RNA |
Reaction Speed | Fast or Standard |
Reagent Type | Cell Lysis |
Green Features | Fewer resources used and less waste, Less hazardous |
For Use With (Application) | Gene Expression |
PCR Method | 1-step RT-qPCR |
Product Line | Cells-to-CT™, TaqMan™ |
Detection Method | Primer-probe |
Thermo Scientific™ Phusion Human Specimen Direct PCR Kit
Perform PCR directly from human samples including buccal swabs, saliva, hair, and fingernails without DNA purification.
Invitrogen™ Power SYBR™ Green Cells-to-CT™ Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Ambion™ Power SYBR™ Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.
Shipping Condition | Dry Ice |
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Purity or Quality Grade | LD (Low DNA) |
Reverse Transcriptase | M-MLV |
Purification Time | 5 min. |
Green Features | Fewer resources used and less waste, Less hazardous |
Concentration | 20X |
For Use With (Application) | RT-PCR |
Detection Method | SYBR |
Polymerase | AmpliTaq Gold DNA Polymerase |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7200 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 5700, GeneAmp 9600, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
Sample Type | Cells |
Reaction Speed | Standard |
Label or Dye | SYBR Green |
Hot Start | Built-In Hot Start |
Product Line | Cells-to-CT™, SYBR™ |
Invitrogen™ ChargeSwitch™-Pro PCR Clean-Up Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
The ChargeSwitch™-Pro PCR Clean-up Kit purifies high-quality DNA fragments without the use of ethanol or harsh salts known to interfere with enzymatic reactions.
Shipping Condition | Room Temperature |
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Target | DNA from Enzymatic Reactions (e.g. PCR) |
Content And Storage | The ChargeSwitch™-Pro PCR Clean-up Kit contains columns, collection tubes, recovery tubes, binding buffer, wash buffer, and elution buffer. |
Format | Kit |
Product Type | PCR Clean-Up Kit |
Sample Type | PCR Products |
Isolation Technology | ChargeSwitch™ Chemistry |
Green Features | Fewer resources used and less waste, Less hazardous |
For Use With (Application) | Next-Generation Sequencing (NGS),Cloning,Standard PCR |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Product Line | ChargeSwitch™ |
Verso™ 1-Step SYBR Green-Based Detection RT-qPCR Kits
Thermo Scientific™ Verso™ 1-Step RT-qPCR Kits have been specially developed to quantify RNA in a convenient one-step assay.
Invitrogen™ Single Cell Lysis Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Single-cell analysis has the potential to transform life science research. However, detecting and quantitating the minute amounts of RNA contained in single cells remains a significant challenge.
Thermo Scientific™ Maxima™ First Strand cDNA Synthesis Kit Reaction Mix (5X)
Thermo Scientific™ Maxima™ 5X Reaction Mix is a component of the Maxima First Strand cDNA Synthesis Kits for RT-qPCR (K1671/K1672/K1641/K1642) and may be purchased separately. The 5X Reaction Mix is comprised of reaction buffer, dNTPs, oligo (dT)18 and random hexamer primers.
Applied Biosystems™ SNaPshot™ Multiplex Kit
The SNaPshot™ Multiplex Kit is part of the SNaPshot™ Multiplex System. The SNaPshot™ Multiplex System primer extension-based method enables multiplexing up to 10 single nucleotide polymorphisms (SNPs).
Product Type | Multiplex Kit |
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For Use With (Equipment) | 3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers, SeqStudio Genetic Analyzer, 3730/3730xl Genetic Analyzers |
Sample Type | DNA |
Technique | SNP Genotyping (Fragment Analysis-Based), Primer Extension |
Product Line | SNaPshot™ |
Detection Method | Primer-probe |
Invitrogen™ TaqMan™ Cells-to-CT™ Express Lysis Reagents
This TaqMan Cells-to-CT Express Lysis Reagents product is a convenient, large volume offering of the cell lysis reagents used in our TaqMan Cells-to-CT Express Kit.
Invitrogen™ PureLink™ Pro 96 PCR Purification Kit
The PureLink™ Pro 96 PCR Purification Kit combines advanced silica plate extraction chemistry with an optimized 96-well plate design for manual or automated processing of PCR cleanup in as little as 20 minutes.