Thermo Scientific™ Phire Plant Direct PCR Master Mix

Product Code. 15255156

Brand: Thermo Scientific™ F160S

Description

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant material like complex polysaccharides, polyphenols, and others.

Phire Plant Direct PCR Master Mix has been tested with leaves and seeds from a wide variety of plant species as well as fungi, bacteria, and yeast. The kit includes a complete set of optimized reagents and detailed protocols, making it an ideal choice for amplification of plant DNA.

Features

• Direct PCR allows sample to be added directly to PCR reaction, reducing time-consuming and expensive DNA purification steps
• Available Dilution & Storage protocol allows several PCR reactions from one sample
• Specially engineered Phire Hot Start II DNA Polymerase for extremely short PCR protocol times
• Master mix format with premixed gel loading dye minimizes risk of cross-contamination, reduces sample handling, and allows direct loading on gel

Applications

• Genotyping
• Transgene detection
• Knockout analysis
• Sequencing

Notes

• Annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.

Specifications

• Concentration: 2X
• Content And Storage: • 2X Phire Plant Direct PCR Master Mix, 2 x 1.25 mL
  • Dilution Buffer, 1 x 5 mL
  • 25 μM Control Primer Mix, 40 μL
  • Nuclease-free water, 2 x 1.25 mL
  • O'GeneRuler Express DNA Ladder, 50 μg (sufficient for 100 applications)
  
  Store at -20°C.
• GC-Rich PCR Performance: Medium
• PCR Method: Direct PCR
• Polymerase: Phire Hot Start II DNA Polymerase
• Reaction Speed: Fast
• Includes Label or Dye: Yes
• Product Type: Direct PCR Master Mix
• Quantity: 250 reactions
• Shipping Condition: Wet or Dry Ice
• For Use With (Application): Genotyping, Hot-start PCR
• Hot Start: Built-In Hot Start
• No. of Reactions: 250 Reactions
• Overhang: Blunt
• Reaction Format: SuperMix or Master Mix
• Sample Type: Plant
• Starting Material: DNA

For Research Use Only. Not for use in diagnostic procedures.