Applied Biosystems™ SNaPshot™ Multiplex Kit

Product Code.	No. of Reactions	unitSize
15809766	100 Reactions	Each
15909182	100 Reactions	100 reactions
15999172	100 Reactions, with protocol	100 reactions
15879756	100 Reactions, with protocol	Each
16355282	1000 Reactions	1000 reactions
15819766	1000 Reactions	Each
16365282	5000 Reactions	5000 reactions
15829766	5000 Reactions	Each

Product Code. 15809766 Supplier Applied Biosystems™ Supplier No. 4323159

Description

Includes

SNaPshot™ Multiplex Ready Reaction Mix (for 100 reactions), SNaPshot™ Multiplex Control Primer Mix (30μL), SNaPshot™ Multiplex Control Template (60μL)

The SNaPshot™ Multiplex Kit is part of the SNaPshot™ Multiplex System. The SNaPshot™ Multiplex System primer extension-based method enables multiplexing up to 10 single nucleotide polymorphisms (SNPs).

• Interrogate up to 10 SNPs from different amplicons in a single base extension reaction
• Separate SNP loci that differ by a single base pair
• Increased sensitivity compared to standard sequencing allows detection of minor variants (e.g. somatic mutations) in a test sample
• Reduce the complexity of your experiments with this easy to use and cost-efficient genotyping system
• A large body of scientific literature demonstrates the utility for genotyping, mutation detection and mapping

Order Info

Each

Specifications

• Content And Storage: Contains:
  • SNaPshot™ Multiplex Ready Reaction Mix (for 100 reactions)
  • SNaPshot™ Multiplex Control Primer Mix (30 μl)
  • SNaPshot™ Multiplex Control Template (60 μl)
  
  Store at -15°C to -25°C in a constant-temperature freezer.
• Description: SNaPshot™ Multiplex Kit, 100 rxns, Capillary Electrophoresis Detection Method, DNA Sample Type, AmpliTaq™ DNA Polymerase, SNPs (Known) Genotyping Target, Multiplexing High Throughput Compatibility, Unlabelled Oligos Primer-Probe Compatibility
• Detection Method: Primer-probe
• Format: Tube
• Polymerase: AmpliTaq DNA Polymerase
• Reaction Speed: 8 hr.
• Technique: SNP Genotyping (Fragment Analysis-Based), Primer Extension
• For Use With (Equipment): 3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers, SeqStudio Genetic Analyzer, 3730/3730xl Genetic Analyzers
• High-throughput Compatibility: Multiplexing
• Product Line: SNaPshot
• Product Type: Multiplex Kit
• Quantity: 100 reactions
• Run Time: 8 hr.
• Shipping Condition: Dry Ice
• Software: GeneMapper™ Software
• Species: All Species
• Target: SNPs (Known)
• For Use With (Application): Genotyping
• Hot Start: No
• No. of Reactions: 100 Reactions
• Sample Type: DNA

Frequently Asked Questions (FAQs)

How many SNPs can the SNaPshot Multiplex kit analyze?

The SNaPshot Multiplex Kis is designed to interrogate up to 10 single nucleotide polymorphisms (SNPs) at known locations on 1-10 templates in a single tube.

I am observing unexpected peaks in my SNaPshot data. Why?

The extra peaks could be due to incomplete removal of PCR primers, incomplete removal of dNTPs from the PCR reaction, or incomplete removal of the fluorescently labeled ddNTPS from the SNaPshot reaction. Residual PCR primers and dNTPs will both participate in the SNaPshot reaction. For the PCR purification, please use fresh SAP and Exo1 or use another method of PCR purification. To address incomplete removal of fluorescently labeled ddNTPS, use fresh CIP or SAP.

If my product is a single-base extension of my primer, shouldn't my products be primer length +1?

Due to the influence of the dye on the mobility shift of the DNA fragments, the reported sizes will differ by a few bases from the actual sizes. This is particularly true with the shorter fragments as the relative contribution of the dye is greater. It is also recommended that you have a tail on the primer with a 5-7 bp difference between them to further separate the samples so there is no overlap.

My SNaPshot Multiplex Kit control reaction does not match the sizes given in the protocol. Is this normal?

This is normal as the SNaPshot control shown in the manual is from a 3100 Genetic Analyzer and POP-4 polymer. Dye-labeled DNA fragments can yield different sizes when run with a different instrument, polymer type, capillary array length, or size standard. Although the control sizes may not match that in the protocol, once the size has been established, the precision, or reproducibility will be consistent for a given fragment.

For Research Use Only. Not for use in diagnostic procedures.