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Invitrogen™ Dynabeads™ Protein A for Immunoprecipitation

Dynabeads Protein A beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein A (∼45 kDa) covalently coupled to the surface.

Pricing and Availability
Specifications

Magnetic Affinity Separation Particles

Shipping Condition	Ambient temperature
Content And Storage	Dynabeads Protein A are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN₃ Store at 2–8°C.
Purity or Quality Grade	For Research Use Only
Description	Recombinant Protein A covalently bound to Dynabeads
Surface Functionality	Protein A
Material	Polystyrene
Concentration	30 mg/mL
For Use With (Application)	Immunoprecipitation
High-throughput Compatibility	High-throughput Compatible
Ligand Type	Protein A
Type	Superparamagnetic Beads
Target	Antibodies
Format	Beads in suspension
For Use With (Equipment)	KingFisher™ Sample Purification System, DynaMag™ magnets
Diameter (Metric)	2.8 μm
Certifications/Compliance	ISO9001 and ISO13485
Uniformity	Monosized 2.8 mm (CV <5%)
Product Line	Dynabeads

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Thermo Scientific™ Halt™ Phosphatase Inhibitor Single-Use Cocktail

Stabilize serine, threonine and tyrosine phosphorylation of proteins in cells and tissues with this cocktail of phosphatase inhibitors.

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Specifications

Protease Inhibitors and Phosphatase Inhibitors

Content And Storage	Upon receipt store product at 4°C. Do not freeze.
Inhibitors	Phosphatase Inhibitor
Form	Liquid
Product Type	Phosphatase Inhibitor Cocktail
Reagent Type	Phosphatase Inhibitor
Product Line	Halt

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Thermo Scientific™ Pierce™ Centrifuge Columns

Pack these empty 0.5 to 10 mL spin columns with agarose beads to perform small-scale affinity purification methods in 15- or 50-mL conical microcentrifuge tubes.

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Specifications

Spin Columns and Gravity-flow Columns

Content And Storage	Store at room temperature.
Column Type	Empty Column, Polyethylene Filter
Product Line	Pierce

Thermo Scientific™ Brij™-35, 30% Solution

Stabilized Brij-35 detergent provided as 10% and 30% solutions for use in various protein methods including cell lysis and HPLC.

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Cell Lysis Detergents

Thermo Scientific™ Lysozyme Solution (50 mg/mL)

Increase protein or nucleic acid extraction efficiency with this enzyme that is characterized by the ability to break down the bacterial cell wall.

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Cell Lysis Enzymes

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Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

Pricing and Availability
Specifications

Protease Inhibitors and Phosphatase Inhibitors

Buffer	Lysis Buffer
Content And Storage	Upon receipt store at 4°C
Inhibitors	Protease Inhibitor, Phosphatase Inhibitor
Form	Liquid
Product Type	Protease and Phosphatase Inhibitor Cocktail
Reagent Type	Protease Inhibitor, Phosphatase Inhibitor
Product Line	Halt

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Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells

Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.

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Protein Purification Reagents and Kits

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Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 0.2 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

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Protein Purification Reagents and Kits

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Thermo Scientific™ Pierce™ High Capacity Streptavidin Agarose

Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.

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Specifications

Biotin-Binding Affinity Resins and Beads

Particle Size	45 to 165 μm
Stationary Phase	Streptavidin
Type	Agarose
Target	Biotin-Tagged Molecule
Content And Storage	Upon receipt store at 4°C. Do not freeze the resin. Product is shipped at ambient temperature.
Format	Bottle
Protein Form	Recombinant
Column Type	Affinity, Agarose Resin
Sufficient For	Binding ≥/= 100 μg Biotin/mL Resin (≥/= 10 mg Biotinylated BSA/mL Resin)
Product Line	Pierce

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Thermo Scientific™ Pierce™ Anti-HA Magnetic Beads

Perform immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.

Pricing and Availability
Specifications

Magnetic Affinity Separation Particles

Particle Size	1 μm
Ligand Type	Antibody
Type	Magnetic Affinity Bead
Target	HA
Content And Storage	Store at 4°C.
Protein Form	Recombinant
Form	Liquid
Concentration	Slurry: 10mg/mL, 1% solids
Product Line	Pierce

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Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

Pricing and Availability
Specifications

Protease Inhibitors and Phosphatase Inhibitors

Buffer	Lysis Buffer
Inhibitors	Protease Inhibitor, Phosphatase Inhibitor
Description	Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-Free
Form	Liquid
Product Type	Protease and Phosphatase Inhibitor Cocktail
Reagent Type	Protease Inhibitor, Phosphatase Inhibitor
Product Line	Halt

Invitrogen™ Dynabeads™ His-Tag Isolation and Pulldown

Dynabeads™ His-Tag Isolation and Pulldown beads are 1-μm monosized, monodispersed, superparamagnetic beads with an NTA-Cobalt-based ligand which has a high affinity towards His-tags and similar structures on molecular targets.

Pricing and Availability
Specifications

Magnetic Affinity Separation Particles

Content And Storage	Dynabeads His-Tag Isolation and Pulldown beads are supplied in 20% ethanol. Store at 2°C to 8°C.
Final Product Type	His-Tagged Protein
Color	Brown
Binding Property	1.6 mg of a 28 kDa histidine-tagged protein/mL (40 mg) beads
Material	Magnetic Polystyrene
Concentration	40 mg/mL
For Use With (Application)	Isolation of His-tagged Recombinant Proteins
High-throughput Compatibility	Yes
Regulatory Status	For Research Use Only
Ligand Type	Nitrilotriacetic acid (NTA)
Type	Magnetic Beads
Target	His-tagged proteins
Format	Magnetic beads
For Use With (Equipment)	KingFisher Sample Purification System, DynaMag magnets
Sample Type	Cell Cultures, Tissue Digests, Intact Proteins, Cell Lysates, Cell Extracts
Diameter (Metric)	1 μm
Certifications/Compliance	ISO9001 and ISO13485
Shelf Life	36 months from date of manufacture
Product Line	Dynabeads

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Thermo Scientific™ Pierce™ Disposable Columns, 2 mL

Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques.

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Spin Columns and Gravity-flow Columns

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Thermo Scientific™ B-PER™ Bacterial Protein Extraction Reagent

Efficiently lyse E.coli and other bacterial cells and gently extract native proteins with these nonionic detergent solutions.

Pricing and Availability
Specifications

Protein Extraction

Description	B-PER Bacterial Protein Extraction Reagent
Product Type	Bacterial Protein Extraction Reagent
Product Line	B-PER

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Thermo Scientific™ Pierce™ Anti-DYKDDDDK Magnetic Agarose

Thermo Scientific™ Pierce™ Anti-DYKDDDK Magnetic Agarose Beads provide a fast, convenient method for purification of DYKDDDK-tagged recombinant proteins

Pricing and Availability
Specifications

Protein Purification Reagents and Kits

dimMatrix	Magnetic Agarose

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