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Thermo Scientific™ CloneJET PCR Cloning Kit
Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.
| Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
|---|---|
| Format | Kit |
| Sample Type | PCR Products |
| Cloning Method | Restriction Enzyme⁄ MCS, Blunt PCR |
| For Use With (Application) | PCR Cloning |
| Vector | pJET1.2/blunt |
| Product Line | CloneJET |
Invitrogen™ GeneArt™ Gibson Assembly HiFi Master Mix
GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.
| Shipping Condition | Ambient, Wet Ice, Dry Ice |
|---|---|
| Content And Storage | Includes master mix, positive control, and water and accommodates the use of your own competent cells. |
| Workflow Step | DNA Assembly |
| Number of Fragments | Up to 6 fragments |
| Product Type | HiFi Master Mix |
| Control Type | Positive Control |
| Cloning Method | Seamless cloning |
| For Use With (Application) | Seamless Cloning & Genetic Assembly |
| Product Line | GENEART™ |
Thermo Scientific™ pUC18 DNA
Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.
Thermo Scientific™ Rapid DNA Ligation Kit
Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.
| Product Type | Rapid DNA Ligation Kit |
|---|---|
| Enzyme | DNA Ligase |
Invitrogen™ GeneArt™ Gibson Assembly EX Master Mix
GeneArt Gibson Assembly EX Master Mix enables DNA assembly via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction.
| Shipping Condition | Dry Ice |
|---|---|
| Content And Storage | Includes master mix, positive control, and water and accommodates the use of your own competent cells. |
| Workflow Step | DNA Assembly |
| Number of Fragments | Up to 15 fragments |
| Product Type | Master Mix |
| Control Type | Positive Control |
| Cloning Method | Seamless cloning |
| For Use With (Application) | Seamless Cloning & Genetic Assembly |
| Product Line | GENEART™ |
Thermo Scientific™ Phusion™ Site-Directed Mutagenesis Kit with DH10B Competent Cells
A versatile and efficient tool for introducing point mutations, insertions, or deletions into any type of plasmid DNA. With this kit the entire plasmid is amplified using phosphorylated primers that introduce the desired changes.
Thermo Scientific™ Lambda DNA (dam–, dcm–)
Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.
Thermo Scientific™ pUC19 DNA
Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.
Thermo Scientific™ phiX174 RF1 DNA
Prepare DNA molecular weight standards with Thermo Scientific™ phiX174 RF1 DNA, a 5386 bp double-stranded closed circular plasmid.
Thermo Scientific™ Fast DNA End Repair Kit
Perform blunting and phosphorylation of DNA ends in just 5 minutes for subsequent use in blunt-end ligation with this kit.
Invitrogen™ GeneArt™ Gibson Assembly EX Cloning Kit, electrocompetent cells
The GeneArt Gibson Assembly EX Cloning Kit enables DNA assembly via a technique that allows up to 15 overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction.
Thermo Scientific™ aLICator LIC Cloning and Expression Kit 3 (C-terminal His-tag)
Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.
Thermo Scientific™ aLICator LIC Cloning and Expression Set 2 (All-in-One/WQ)
Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.
Thermo Scientific™ pTZ19R DNA
Perform DNA cloning, dideoxy DNA sequencing, in vitro mutagenesis, and in vitro transcription in one system with this double-stranded closed circular high copy phagemid DNA.
Invitrogen™ GeneArt™ Gibson Assembly HiFi Cloning Kit, electrocompetent cells
The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.